Supplemental Smartamine M in higher-energy diets during the prepartal period improves hepatic biomarkers of health and oxidative status in Holstein cows  

作　　者：Mario Vailati-Riboni Johan S.Osorio Erminio Trevisi Daniel Luchini Juan J.Loor 

机构地区：[1]Mammalian Nutri Physio Genomics,Department of Animal Sciences and Division of Nutritional Sciences,University of Illinois,Urbana,IL 61801,USA [2]Istituto di Zootecnica Facolt àdi Scienze Agrarie,Alimentari e Ambientali,Università Cattolica del Sacro Cuore,29122 Piacenza,Italy [3]Adisseo NA,Alpharetta,GA 30022,USA. [4]Dairy and Food Science Department,South Dakota State University,1111 College Ave,113H Alfred DairyScience Hall,Brookings SD 57007,USA

出　　处：《Journal of Animal Science and Biotechnology》2017年第3期624-635,共12页畜牧与生物技术杂志（英文版）

基　　金：Financial support for the research was provided in part by Adisseo(Commentry,France);Hatch funds under project ILLU-538–914,National Institute of Food and Agriculture,Washington,DC,USA

摘　　要：Background： Feeding higher-energy prepartum is a common practice in the dairy industry. However, recent data underscore how it could reduce performance, deepen negative energy balance, and augment inflammation and oxidative stress in fresh cows. We tested the effectiveness of rumen-protected methionine in preventing the negative effect of feeding a higher-energy prepartum. Multiparous Holstein cows were fed a control lower-energy diet（CON, 1.24 Mcal/kg DM; high-straw） during the whole dry period（-50 d）, or were switched to a higher-energy（OVE, 1.54 Mcal/kg DM）, or OVE plus Smartamine M（OVE ＋ SM; Adisseo NA） during the last 21 d before calving.Afterwards cows received the same lactation diet（1.75 Mcal/kg DM）. Smartamine M was top-dressed on the OVE diet（0.07% of DM） from -21 through 30 d in milk（DIM）. Liver samples were obtained via percutaneous biopsy at -10, 7 and 21 DIM. Expression of genes associated with energy and lipid metabolism, hepatokines, methionine cycle, antioxidant capacity and inflammation was measured.Results： Postpartal dry matter intake, milk yield, and energy-corrected milk were higher in CON and OVE ＋ SM compared with OVE. Furthermore, milk protein and fat percentages were greater in OVE ＋ SM compared with CON and OVE. Expression of the gluconeogenic gene PCK1 and the lipid-metabolism transcription regulator PPARA was again greater with CON and OVE ＋ SM compared with OVE. Expression of the lipoprotein synthesis enzyme MTTP was lower in OVE ＋ SM than CON or OVE. Similarly, the hepatokine FGF21, which correlates with severity of negative energy balance, was increased postpartum only in OVE compared to the other two groups. These results indicate greater liver metabolism and functions to support a greater production in OVE ＋ SM. At 7 DIM, the enzyme GSR involved in the synthesis of glutathione tended to be upregulated in OVE than CON-fed cows, suggesting a greater antioxidant demand in overfed cows. Feeding OVE ＋ SM resulted in lower similar expressioBackground： Feeding higher-energy prepartum is a common practice in the dairy industry. However, recent data underscore how it could reduce performance, deepen negative energy balance, and augment inflammation and oxidative stress in fresh cows. We tested the effectiveness of rumen-protected methionine in preventing the negative effect of feeding a higher-energy prepartum. Multiparous Holstein cows were fed a control lower-energy diet（CON, 1.24 Mcal/kg DM; high-straw） during the whole dry period（-50 d）, or were switched to a higher-energy（OVE, 1.54 Mcal/kg DM）, or OVE plus Smartamine M（OVE ＋ SM; Adisseo NA） during the last 21 d before calving.Afterwards cows received the same lactation diet（1.75 Mcal/kg DM）. Smartamine M was top-dressed on the OVE diet（0.07% of DM） from -21 through 30 d in milk（DIM）. Liver samples were obtained via percutaneous biopsy at -10, 7 and 21 DIM. Expression of genes associated with energy and lipid metabolism, hepatokines, methionine cycle, antioxidant capacity and inflammation was measured.Results： Postpartal dry matter intake, milk yield, and energy-corrected milk were higher in CON and OVE ＋ SM compared with OVE. Furthermore, milk protein and fat percentages were greater in OVE ＋ SM compared with CON and OVE. Expression of the gluconeogenic gene PCK1 and the lipid-metabolism transcription regulator PPARA was again greater with CON and OVE ＋ SM compared with OVE. Expression of the lipoprotein synthesis enzyme MTTP was lower in OVE ＋ SM than CON or OVE. Similarly, the hepatokine FGF21, which correlates with severity of negative energy balance, was increased postpartum only in OVE compared to the other two groups. These results indicate greater liver metabolism and functions to support a greater production in OVE ＋ SM. At 7 DIM, the enzyme GSR involved in the synthesis of glutathione tended to be upregulated in OVE than CON-fed cows, suggesting a greater antioxidant demand in overfed cows. Feeding OVE ＋ SM resulted in lower similar expressio

关 键 词：Energy Methionine Nutrigenomics Transition period 

分 类 号：S823.5[农业科学—畜牧学]