紫外-可见分光光度法测大黄不同炮制品总蒽醌含量  

Determination of Total Anthraquinone in Different Processed Products of Rhubarb by UV Visible Spectrophotometry

作　　者：魏江存 陈勇[1] 谢臻[1] 李金洲[1] 韦乐亮 邓丽红[1] 许爱德[1] 兰帆[1] 胡小兰[1] 

机构地区：[1]广西中医药大学,广西南宁530020

出　　处：《广州化工》2017年第17期126-128,共3页GuangZhou Chemical Industry

基　　金：国家自然科学基金(No.81360524);壮瑶药协同创新中心(No.桂教科研[2013]20号);广西中医药大学第一附属医院青年科学基金(No.GZYQJ13);广西中医药大学科研创新项目(No.YJS201625);广西壮瑶药重点实验室(No.桂科基字[2014]32号);广西重点学科壮药学(No.桂教科研[2013]16号);广西八桂学者中药创新理论与药效研究(No.J13162)

摘　　要：建立紫外分光光度法测定大黄不同炮制品中总蒽醌的含量,以大黄素为对照品,浓度为1%的醋酸镁甲醇溶液对其进行显色,在516 nm波长处测定吸光度,测定大黄不同炮制品总蒽醌的含量,比较其含量差异,其回归方程为:y=0.0452x+0.0015,R^2=0.9991,大黄素在浓度为0.9823.52μg/m L内有良好的线性范围,且准确性高、重复性好、检测结果稳定。该方法揭示中药大黄炮制前后总蒽醌的含量变化。该方法可作为大黄不同炮制品的质量控制方法,为今后综合开发利用中药大黄资源提供参考依据。The content of total anthraquinone in different processed products of rhubarb was determined by ultraviolet speetrophotometry. With emodin as the reference substance, magnesium acetate methanol solution with concentration of 1% had coloration to total anthraquinone. The absorbance was measured at the wavelength of 516 nm. The regression equation was y=0. 0452x＋0. 0015, R2 =0. 9991, emodin had a good linear range in the concentration of 0. 98 ~23.52 μg/mL, and the accuracy was high, repeatability was good, and the detection results were stable. The content of total anthraquinone in different processed products of rhubarb was determined, and the content of total anthraquinone was changed before and after the processing of rhubarb. This method can be used to control the quality of different processed products of rhubarb, it provided a reference for the comprehensive development and utilization of Chinese rhubarb resources in the future.

关 键 词：大黄 总蒽醌 紫外分光光度法 

分 类 号：R917[医药卫生—药物分析学]