地黄叶多糖的分离纯化、结构分析及免疫功能研究  

Characterization and Immunity Activity of Polysaccharides from the Leaves of Rehmannia glutinosa Libosch

作　　者：曾敏[1] 徐惠芳[1] 余江琴 

机构地区：[1]武汉市中医医院综合药学部,湖北武汉430014

出　　处：《现代中药研究与实践》2017年第2期27-30,共4页Research and Practice on Chinese Medicines

摘　　要：目的从地黄叶中分离纯化多糖,并对其进行结构和活性的研究。方法采用DEAE-52树脂和葡聚糖凝胶对地黄叶多糖进行分离纯化,并对其结构进行表征,最后利用脾细胞增殖试验研究了地黄叶多糖的免疫功能。结果从地黄叶中分离纯化得到了三种均一多糖(RGLPW,RGLPS1和RGLPS2),其中RGLPW为中性多糖,RGLPS1和RGLPS2为酸性多糖。免疫学活性实验表明,RGLPW和RGLPS2有着明显促进脾细胞增殖作用。结论 RGLPW和RGLPS2具有增强机体免疫作用,可以作为功能性食品或者药品添加剂。Objective Polysaccharides from the leaves of Rehmannia glutinosa Libosch were isolated and purified, and the properties of their immunological activities were studied. Methods Polysaccharides from the leaves of Rehmannia glutinosa Libosch were isolated by DEAE-52 and sephadex gel cellulose chromatography and their structures and immunological activities were characterized using physicochemical and spectral methods. Results One neutral polysaccharide RGLPW and two acidic polysaccharides RGLPS1 and RGLPS2 were isolated. In vitro, the data suggested that polysaccharides RGLPW and RGLPS2 had a potential in promoting the proliferation of spleen lymphocytes. Conclusion These findings implied that RGLPW, RGLPS1 or RGLPS2 might be a candidate for developing immunopotentiating agents in food and pharmaceutical industries.

关 键 词：地黄叶 多糖 免疫活性 

分 类 号：R285[医药卫生—中药学]