Adrenomedullin promotes the odontogenic differentiation of dental pulp stem cells through CREB/BMP2 signaling pathway  

作　　者：Qiang Zhu Jianyong Gao Gang Tian Zhen Tang Yinghui Tan 

机构地区：[1]Department of Stomatology, Changhai Hospital, the Second Military Medical University, Shanghai 200433, China [2]Department of Stomatology, Xinqiao Hospital, the Third Military Medical University, Chongqing 400037, China

出　　处：《Acta Biochimica et Biophysica Sinica》2017年第7期609-616,共8页生物化学与生物物理学报（英文版）

摘　　要：Adrenomedullin （AM） could promote the proliferation, the odontogenic differentiation and inhibit the apoptosis of dental pulp stem cells （DPSCs）. AM in combination with DPSCs may be an effective strategy for pulp repair. However, there was no report on the mechanisms of AM in the odontogenic differentiation of DPSCs. The aim of this study is to investigate the molecular mechanisms through which AM promotes the odontogenic differentiation of DPSCs. Freshly extracted wisdom teeth were obtained from 27 patients. Cells at passage 3 to passage 5 were used in this study. DPSCs were treated with or without 10-7M AM in Dulbecco＇s modified Eagle＇s medium culture, and then the accumulated calcium deposition was analyzed after 21 days by using alizarin red S staining. Odontogenic differentiation markers were determined by western blot analysis and quantitative real-time PCR. Western blot analysis results showed that AM had the capability of promoting the odontogenic differentiation of DPSCs and AM could enhance the phosphorylation of CREB and up-regulate the expression of BMP2. H89 is a CREB inhibitor which can inhibit the odontogenic differentiation of DPSCs through inhibiting the phosphorylation of CREB. Noggin could inhibit the odontogenic differentiation of DPSCs through inhibiting the activity of BMP2. These results indicated that AM could promote the odontogenic differentiation of DPSCs by upregulating the expression of BMP2 through the CREB signaling pathway.Adrenomedullin （AM） could promote the proliferation, the odontogenic differentiation and inhibit the apoptosis of dental pulp stem cells （DPSCs）. AM in combination with DPSCs may be an effective strategy for pulp repair. However, there was no report on the mechanisms of AM in the odontogenic differentiation of DPSCs. The aim of this study is to investigate the molecular mechanisms through which AM promotes the odontogenic differentiation of DPSCs. Freshly extracted wisdom teeth were obtained from 27 patients. Cells at passage 3 to passage 5 were used in this study. DPSCs were treated with or without 10-7M AM in Dulbecco＇s modified Eagle＇s medium culture, and then the accumulated calcium deposition was analyzed after 21 days by using alizarin red S staining. Odontogenic differentiation markers were determined by western blot analysis and quantitative real-time PCR. Western blot analysis results showed that AM had the capability of promoting the odontogenic differentiation of DPSCs and AM could enhance the phosphorylation of CREB and up-regulate the expression of BMP2. H89 is a CREB inhibitor which can inhibit the odontogenic differentiation of DPSCs through inhibiting the phosphorylation of CREB. Noggin could inhibit the odontogenic differentiation of DPSCs through inhibiting the activity of BMP2. These results indicated that AM could promote the odontogenic differentiation of DPSCs by upregulating the expression of BMP2 through the CREB signaling pathway.

关 键 词：ADRENOMEDULLIN dental pulp stem cell odontegenic differentiation 

分 类 号：Q813.11[生物学—生物工程] Q959.837