两种微载体培养鼠肝细胞对低温保存后的影响  

Infl uence of Two Culture Configuration on the Post-Cryopreservation of Rat Hepatocytes

作　　者：杨波[1] 庄灵霞 刘宝林[1] 王政[1] 许可[1] 

机构地区：[1]上海理工大学医疗器械与食品学院,上海200093

出　　处：《中国细胞生物学学报》2017年第8期1008-1014,共7页Chinese Journal of Cell Biology

基　　金：国家自然科学基金(批准号:51076108)资助的课题~~

摘　　要：为了研究细胞培养方式对低温保存后细胞功能的影响,将大鼠肝细胞接种至两种不同微载体(实体微载体Cytodex、多孔微载体Cytopore),微重力高密度培养后于–80°C冻存(冻存速率是1°C/min,5%DMSO+0.4 mol/L山梨糖醇)。2周后复温细胞与载体材料,用显微镜观察细胞生长形态,计算细胞活力情况,并通过细胞代谢功能指标葡萄糖、白蛋白、尿素等的测定,反映细胞在两种载体培养和低温保存后的生长和代谢情况。结果表明,在细胞培养期间,Cytopore的MTT值、代谢指标值是Cytodex的1~1.5倍,低温保存后,Cytopore的各项指标与低温保存前差异不显著,而Cytodex的代谢指标差异显著。因此,大鼠肝细胞在微载体Cytopore的高密度培养及低温保存比Cytodex更有利于细胞的生长和代谢。In order to study the infl uence of cell culture configuration on cell function after cryopreservation, we propose to administer rat hepatocytes to two different microcarriers（entity microcarrier Cytodex, porous microcarrier Cytopore） and cryoperserved in –80 °C after being cultured in minute gravity and high density（cooling at 1 °C/min using 5% DMSO＋0.4 mol/L sorbitol）. After two weeks of cryopreservation, the cells and microcarrier materials were thawed. Through the cell metabolism index determination of glucose, albumin, urea and the observation of the cell growth and cell vitality to show the situation of the growth and metabolism cells cultured in two kinds of carriers. The result demonstrated that during the time of cell culture, the MTT value and metabolic index of Cytopore were about 1-1.5 times compared to Cytodex. Furthermore, there were no significant difference among the each indicators of Cytopore after cryopreservation, while the indicators of Cytodex were quite different. Hence, the cultivation of rat liver cells in carrier Cytopore with high density and cryopresreved is better for the growth, proliferation and metabolism of cells than that in the carrier Cytodex.

关 键 词：大鼠肝细胞 Cytodex Cytopore 高密度培养 低温保存 

分 类 号：Q813.1[生物学—生物工程]