不同孔径的纳米多孔氧化铝膜对细胞迁移的影响  

The Effect of the Nanoporous Alumina Films with Different Pore Diameters on the Cell Migration

作　　者：安郁宽[1] 闫鹏[1] 徐艳岩[2] 

机构地区：[1]滨州医学院基础学院,烟台264003 [2]滨州医学院药学院,烟台264003

出　　处：《中国细胞生物学学报》2017年第8期1084-1090,共7页Chinese Journal of Cell Biology

基　　金：山东科技发展计划(政策引导类)(批准号:2011YD02094);国家自然科学基金(批准号:51401031)资助的课题~~

摘　　要：为了研究不同孔径的纳米多孔阳极氧化铝膜对细胞迁移的影响,采用扫描电镜和X射线能谱仪分别检测不同孔径纳米氧化铝膜微观结构与元素组成;将Hela细胞常规培养于激光共聚焦皿内,分为对照组、40~70 nm孔径组和200~300 nm孔径组三组,进行划痕实验;将皿放入活细胞工作站随机选取多个观测点,连续观测24 h;从迁移面积、边缘迁移距离和单细胞迁移速度等方面进行细胞迁移分析;用扫描电镜对细胞在两种膜上生长24 h后的情况进行观测。结果显示,40~70 nm孔径组与对照组相比可加快细胞迁移(P<0.05),200~300 nm孔径组与对照组相比可减缓细胞迁移(P<0.05)。该研究结果表明,纳米氧化铝膜的孔径不同对细胞迁移影响不同,这为临床研究中控制细胞行为材料的设计和研发提供了实验依据。To investigate the effects of the nanoporous anodic alumina（NAA） films with different pore diameters on the Hela cells migration, the Scanning Electron Microscope（SEM） and the Energy Dispersive X-ray Spectroscopy were used to characterize the microcosmic structure and chemical components of the NAA films respectively. The Hela cells were cultured in confocal dishes, divided into three groups（control group, 40-70 nm pore diameter group and 200-300 nm pore diameter group）, to perform wound healing assay, randomly picked several observing points to observe 24 hours continuously in the live cell imaging system. The cell migration analysis was performed in terms of migration area, migration distance of the edge and migration speed of single cell. The growth status of the cells after cultured 24 hours on both films was characterized by the SEM. The results showed that the 40-70 nm pore diameter group was able to speed the cell migration compared to the control group（P〈0.05）, while the 200-300 nm pore diameter group was slower（P〈0.05）. These data suggested that the NAA films with defferent pore sizes had different effects on the cell migration, which might provide the evidence for the design, study and development of the materials controlling cell behavior in the clinical research.

关 键 词：纳米多孔阳极氧化铝膜 活细胞工作站 细胞迁移 划痕实验 

分 类 号：R318.08[医药卫生—生物医学工程]