微生物肥料在不同节肥水平下对烟草根际微生物碳代谢指纹的影响  

Effects of Microbial Fertilizer in the Different Fertilizer-saving Levels on C Metabolic Fingerprint of Tobacco Rizosphere Microorganisms

作　　者：杨旭初[1,2] 龙莉[3] 屠乃美[1] 侯方舟 付小红[1] 

机构地区：[1]湖南农业大学农学院,湖南长沙410128 [2]江西省红壤研究所,江西南昌331717 [3]湖南农业大学教育学院,湖南长沙410128

出　　处：《核农学报》2017年第10期2016-2022,共7页Journal of Nuclear Agricultural Sciences

基　　金：公益性行业(农业)科研专项(201503123-05)

摘　　要：为了探究微生物肥料在节肥条件下对烟草根际微生物功能多样性的影响,应用Biolog-ECO技术,研究了金农肥中肥和德根贝在0%、10%、20%、30%节肥水平下烟草根际微生物碳代谢特征。结果表明,金农肥中肥和德根贝的根际微生物,对4类碳源的利用能力均随节肥量的增加而下降;主成分分析表明,在节肥水平下,金农肥中肥和德根贝的根际微生物对碳源的利用方式不同,金农肥中肥偏好代谢中间产物及次生代谢物,而德根贝偏好糖类、脂肪酸和脂类;金农肥中肥和德根贝的根际微生物群落的丰富度、均匀度、优势度在各节肥水平下均显著高于全量施肥(P<0.05);从减少化肥施用量对根际微生物碳源代谢能力、碳源利用多样性的影响来看,金农肥中肥和德根贝的节肥比例均以不高于20%为宜,且德根贝表现更优。本研究结果为烟草生产上微生物肥料和节肥能力的选择提供了理论依据。In order to explore the effects of microbial fertilizer on functional diversity of rhizosphere microorganisms in fertilizer-saving levels,the Biolog-ECO technology was adopted to study the effect of two microbial fertilizers（Jinnong No. 1 and Degenbei） on carbon metabolism characteristics of rhizosphere microbe functional diversity in 4 fertilizersaving levels（0%,10%,20% and 30%）. The results showed that： the utilization ability of 4 carbon sources were decreased as the amount of chemical fertilizer reduction in groups treated with microbial fertilizer. The principal component analysis showed that there were difference between 2 kinds of microbial fertilizers on utilization of the four carbon sources. The preference of Jinnong No. 1 were intermedia and secondary metabolites,while the preference of Degenbei were glucides,fatty acids and lipids. The richness index,dominance index and evenness index of groups treated with microbial fertilizer were significantly higher than those of CK in 4 fertilizer-saving levels（P〈 0. 05）. The fertilizer-saving proportion of two microbial fertilizers should be less than 20%,and the comprehensive effect of Degenbei was better than Jinnong No. 1 on two aspects of rhizosphere microbial carbon source utilization ability,and metabolic diversity. The results provide a theoretical basis for the selection of microbial fertilizers and fertilizer-saving proportion in tobacco production.

关 键 词：微生物肥料 烟草 根际 碳代谢指纹 

分 类 号：S144[农业科学—肥料学] S572[农业科学—农业基础科学]