热激处理对草菇耐冷性的影响及对hsp100基因的诱导  

The Effect of Heat Treatment on Adaptation of Volvariella volvacea to Low Temperature Stress and on Relative Expression of hsp100 Gene

作　　者：赵妍[1] 杨焕玲[1] 黄金丽[1] 陈明杰[1] 王晨光 任畇霏 马丹丹[1] 

机构地区：[1]国家食用菌工程技术研究中心农业部南方食用菌资源利用重点实验室上海市农业遗传育种重点开放实验室上海市农业科学院食用菌研究所,上海201403 [2]上海百信生物科技有限公司,上海201403

出　　处：《分子植物育种》2017年第8期3036-3041,共6页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31301828);上海市科技兴农重点攻关项目(沪农科攻字2014第7-1-5号)共同资助

摘　　要：热激蛋白是生物体抵御逆境胁迫的重要蛋白,高温胁迫是诱导热激蛋白产生的重要因素,其中Hsp100蛋白可以在高温诱导时大量产生。本研究以草菇菌株V23和VH3为试验材料,首先观察了热激处理对低温胁迫后草菇菌丝恢复生长情况的影响,然后测定了热激处理再进行低温胁迫后草菇hsp100基因的表达量变化。研究结果显示:热激处理显著提高了草菇V23与VH3的恢复生长速度,增加了二者菌丝体中hsp100基因的表达量,有助于增强草菇对低温胁迫的耐受性。Heat shock proteins（HSPs） protect against cellular damage caused by environmental stresses.Hsp100 is one of the HSPs, which are often induced by heat treatment. This experiment was conducted with the mycelia of two Volvariella volvacea strains V23 and VH3. The effect of heat treatment on recovery growth rate of the two strains growing for 24 h was observed. Relative expression of hsp100 gene under low temperature stress in mycelia of V23 and VH3 after being treated at 40℃ for 2 h was also measured. Results showed that heat treatment speeded up the recovery growth rate of V23 and VH3 and increased the relative expression of hsp100 gene under low temperature stress. All these results suggested that heat treatment could be helpful for the adaptation of V.volvacea to low temperature stress.

关 键 词：草菇 热激 低温 hsp100 实时荧光定量PCR 

分 类 号：S646.13[农业科学—蔬菜学]