基于SSR标记的草莓品种DNA指纹图谱的构建及应用  

Construction and Application of DNA Fingerprinting of Strawberry(Fragaria ananassa Duch.) Varieties Based on SSR Markers

作　　者：黄志城[1] 石敬韡 庞夫花[3] 王显生[3] 赵密珍[3] 陈海荣[1] 

机构地区：[1]上海市农业科学院农产品质量标准与检测技术研究所农业部植物新品种测试(上海)分中心,上海201403 [2]江西农业大学,南昌330045 [3]江苏省农业科学院,南京210014

出　　处：《分子植物育种》2017年第8期3097-3116,共20页Molecular Plant Breeding

基　　金：上海市市级农口系统青年人才成长计划(沪农青字2015第1-31号);2016年度农业部品种资源保护项目共同资助

摘　　要：以49份申请品种保护提供的品种和47份收集的草莓已知品种为材料,通过从208对草莓SSR引物中筛选出20对多态性好的核心引物,采用变性聚丙烯酰氨凝胶电泳检测和毛细光电泳检测相结合的方式对96份草莓品种进行标记分析,检测每个标记不同等位变异大小,并为每个等位变异选取了相应的参照品种。结果显示:20对引物共检测出206个等位位点,每对引物检测到等位位点6~15个平均10.3个;共检测到600个带型,每对引物检测到带型数11~58个,平均30个,平均多态性信息含量(polymorphism information content,PIC)为0.80。聚类结果表明,在遗传相似系数为0.96时,该套引物能将所有品种区分开,同一单位选育的品种被优先聚为一类;对历年草莓申请品种选择的近似品种做出合理性评价,并对今年新的部分申请品种推荐更为相近的近似品种,同时本研究中可能存在的"同名异物"和"同物异名"的品种还需基于外观表达性状的观测。In this research, 49 candidate varieties and 47 reference varieties had been analyzed by using 20 SSR primer pairs, with high polymorphisms, which selected from initial 208 SSR primers, through combining the methods of denaturing polyacrylamide gel electrophoresis and capillary electrophoresis, in order to detect the fluorescently labeled PCR-amplified fragments and the size of the alleles. Example varieties corresponding to each allele were assigned. The results showed that a total of 206 polymorphic alleles and 600 polymorphic patterns were revealed by these 20 primer pairs, with a range of 6 to 15 alleles and 11 to 58 patterns, and an average of 10.3alleles and 30 patterns for each primer pair. The mean value of polymorphism information content was 0.80. The result of clustering analysis showed that all varieties were completely differed from each other by using these 20 primer pairs based on the threshold of genetic similarity coefficient at 0.96. Obviously, the varieties came from the same breeders were clustered together. Otherwise, a reasonable assessment on similar variety-selecting for candidate varieties had been made, and some more suitable similar varieties had also been recommended to candidate varieties which were tested at the first time this year. Finally, some‘homonym＇and‘synonym＇varieties were able to exist in this research which needed to do further testing based on morphological characteristics.

关 键 词：草莓 SSR 荧光标记 DNA指纹 

分 类 号：S668.4[农业科学—果树学]