ISSR荧光标记毛细管电泳与谷子品种遗传多样性分析  

Genetic Diversity Analysis with Capillary Electrophoresis Detection with Fluorescent ISSR Markers on Foxtail Millet

作　　者：常玉卉[1] 张艾英[2] 刘盼 张莉[3] 

机构地区：[1]山西省农业科学院高梁研究所,榆次030031 [2]山西省农业科学院谷子研究所,长治046011 [3]山西农业大学农学院,太谷030801

出　　处：《分子植物育种》2017年第8期3302-3308,共7页Molecular Plant Breeding

基　　金：国家自然科学基金项目(31501323);山西省回国留学人员科研资助项目(2016-071)共同资助

摘　　要：谷子源于中国,遗传多样性丰富,近年来,科研人员培育出来了许多新品种,但是,可选用的目标种质资源不多,育种形势不乐观。ISSR荧光标记毛细管电泳法是一种以DNA测序为平台的检测方法,拥有更高的自动化、准确性等一些优点。本实验利用ISSR荧光标记毛细管电泳技术对30个优异的谷子品种进行了遗传多样性分析。ISSR分析结果也表明参试的谷子品种遗传变异丰富,8条ISSR引物扩增出120多条多态性条带,遗传相似系数的变化范围为0.89~0.93。数据处理后聚类结果发现一些地理来源不一致的品种也被聚集在一起,这种情况的出现或许是因为人类的行为所造成的,使得谷子品种间体现出了近亲缘的关系。本实验可以为谷子品种的分类鉴定、谷子优异种质的科学利用及分子标记辅助育种提供理论依据。Foxtail millet, originated from China, is an important crop with high genetic diversity. In recent years,many germplasm were developed, but germplasm resources are still limited. The genetic diversity of 30 foxtail millet germplasms was elevated by capillary electrophoresis detection with fluorescent ISSR markers, which has a higher degree of automation, accuracy and some other advantages. Eight ISSR primers were used to amplify 30 individuals. A total of 120 ISSR bands were obtained by 8 primers. Genetic similarity coefficients were changed from 0.89 to 0.93. The results showed that some individuals from different regions were clustered into the same group indicating that the relationships among these genotypes were close due to domestication and artificial breeding. This study could provide a theoretical base for classification and identification of millet varieties,scientific utilization of millet germplasm and molecular marker assisted breeding.

关 键 词：谷子 ISSR荧光标记 毛细管电泳 遗传多样性 

分 类 号：S515[农业科学—作物学]