多疣壁虎SOCS3A的分子克隆和断尾损伤脊髓中的表达变化研究  

Cloning of SOCS3A from Gekko japonicus and its expression analysis in the spinal cord following tail amputation

作　　者：张薛杰 王文娟[1] 杜楠[1] 宋添铖 王勇军[1] 

机构地区：[1]南通大学神经再生重点实验室,南通226001

出　　处：《南通大学学报（医学版）》2017年第3期192-196,共5页Journal of Nantong University(Medical sciences)

基　　金：江苏省普通高校研究生科研创新计划项目(KYZZ15_0352);国家自然科学基金资助项目(31640042)

摘　　要：目的:克隆多疣壁虎细胞因子信号转导负调控蛋白3A(suppressor of cytokine signaling,SOCS3A)基因的c DNA全长序列,并分析其结构特征;检测SOCS3A蛋白在多疣壁虎断尾损伤脊髓中的表达变化。方法:利用c DNA末端快速扩增(rapid amplification of c DNA ends,RACE)技术克隆得到多疣壁虎SOCS3A的c DNA全长序列;实时荧光定量聚合酶链反应(real-time polymerase chain reaction,RT-PCR)方法检测多疣壁虎断尾脊髓中SOCS3A的表达。结果:克隆得到多疣壁虎SOCS3A的c DNA全长序列。该序列全长1 152 bp,编码202个氨基酸,与爬行动物SOCS3同源性高度保守;多疣壁虎断尾损伤后,SOCS3A在断尾脊髓中的表达先下调随后上调。结论:成功克隆了多疣壁虎SOCS3A的c DNA全长序列,共1 152 bp,编码202个氨基酸。SOCS3A在多疣壁虎断尾再生脊髓中表达升高。Objective： To clone the full length cDNA of suppressor of cytokine signaling 3A（SOCS3A） from Gekko japonicus, and characterize its primary structure; then we will examine its expression changes in the spinal cord following gecko tail amputation. Methods： To obtain the full-length of SOCS3A cDNA from gecko by rapid amplification of cDNA ends（RACE） technique. The expression changes of SOCS3 in the spinal cord were analyzed by real-time polymerase chain reaction （RT- PCR）. Results： The SOCS3A cDNA of gecko was cloned with the full length of 1 152 bp encoding a protein of 202 amino acids. SOCS3A showed a high conservation during the phylogeny, especially among reptiles. The expression of SOCS3A was upregulated in the regenerating spinal cord following gecko tail amputation. Conclusion： The SOCS3A cDNA of gecko was cloned with the full length of 1 152 bp encoding a protein of 202 amino acids. The expression of SOCS3A was upregulated in the regenerating spinal cord following gecko tail amputation.

关 键 词：细胞因子信号转导负调控蛋白3A 分子克隆 脊髓 壁虎 

分 类 号：Q781[生物学—分子生物学]