红掌AaMYB1基因的克隆、表达及异源转化研究  

Gene Cloning,Expression and Ectopic Transformation Analysis of AaMYB1 From Anthurium

作　　者：马广莹[1] 史小华[1] 朱开元[1] 金亮[1] 邹清成[1] 刘慧春[1] 张加强[1] 田丹青[1] 

机构地区：[1]浙江省农业科学院花卉研究开发中心,浙江杭州311202

出　　处：《核农学报》2017年第9期1708-1716,共9页Journal of Nuclear Agricultural Sciences

基　　金：国家自然科学基金(31200527);浙江省农业(林木)新品种选育专项(2016C02056-13)

摘　　要：MYB类转录因子在植物观赏器官色彩形成中发挥关键作用。为了研究红掌中该类转录因子的种类、表达、作用机理等,本研究从红掌中获得了一个MYB转录因子的基因序列,通过反转录多聚酶链式反应(RT-PCR)、生物信息学软件分析、荧光定量PCR检测、构建超表达载体并异源转化烟草等手段,对该转录因子进行了初步研究。结果表明,该转录因子编码基因包含完整编码区,共计912 bp,编码303个氨基酸残基,序列组成与其他物种同源体具有高度相似性;荧光定量分析显示,Aa MYB1在红掌不同组织部位都有表达,但在苞片中表达量最高;获得了12株阳性转化株,形态观察发现转化株营养器官花色素累积程度随基因表达不同而异,但可使所有转化株花器官颜色显著加深。本研究结果为进一步探究MYB转录因子在红掌中调控花色素合成等信息提供了有益参考。MYB transcription factors in ornamental plants play a key role in the formation of color. In order to get insight into the type,expression pattern and functional mechanism of AaMYB1, reverse transcription polymerase chain reaction （RT-PCR） , bioinformatics software analysis, and fluorescence quantitative PCR detection were adopted to investigate this factor above problems. Over-expression vector were constructed and heterologously transformed to tobacco. The results showed that the obtained sequence contained a complete encoding region, which consisted of 912 base pairs, encoded 303 amino acid residues, and shared high similarity with homologous genes in other species. Fluorescence quantitative analysis showed that AaMYB1 expressed in different tissues of Anthurium, but the highest expression level was found in bract. 12 positive transformants were obtained by transgenic technology, and morphological observation indicated that anthocyanin accumulation was different with the level of exogenous gene expression, however, the color of all floral organs from different transformants deepeded significantly compared with the control. The results of this study provided a useful reference for further research on the MYB type transcription factors in Anthurium.

关 键 词：红掌 表达分析 花色苷 转基因 

分 类 号：S682.14[农业科学—观赏园艺]