新法合成纳米银及其对鸟分枝杆菌的抗菌活性研究  

Study on the effect of new method of synthesis of silver nanoparticles on Mycobacterium avium

作　　者：王春花[1] 李维宏[2] 赵慧[1] 穆成[1] 孙蕊[1] 

机构地区：[1]天津市结核病控制中心参比室,300041 [2]山西农业大学环境科学院

出　　处：《中国防痨杂志》2017年第9期1022-1025,共4页Chinese Journal of Antituberculosis

摘　　要：以山楂叶水提取物作为还原剂,采用生物法合成纳米银粒子.采用BACTEC MGIT 960 培养系统对结核分枝杆菌标准株H37Rv和鸟分枝杆菌（ATCC25291）对链霉素、异烟肼、利福平、乙胺丁醇一线抗结核药物,以及纳米银终浓度为0.15、0.30、0.60、1.20 μg/ml等4个剂量组进行药物敏感性试验.发现当pH值为11、浓度为0.5 mmol/L硝酸银溶液29.9 ml与0.1 ml山楂叶水提取物混合,反应条件为80 ℃、水浴15 min时,合成的纳米银粒径为7 （±23%） nm,为最佳合成条件.新法合成纳米银在较低浓度下（0.30 μg/ml）即显示出对鸟分枝杆菌和H37Rv具有抗菌活性.Silver nanoparticles were successfully synthesized from aqueous silver nitrate through a simple green route using the extract of hawthorn leaves as a reducing as well as capping agent.BACTEC MGIT 960 culture systems was used to evaluate the antibacterial activity of silver nanoparticles on Mycobacterium avium （ATCC25291） and H37Rv for streptomycin,isoniazid, rifampicin and ethambutol with the final concentration of silver nanoparticles at 0.15 μg/ml,0.30 μg/ml, 0.60 μg/ml and 1.20 g/ml.The best reaction conditions were pH 11.0, 0.1 ml of the extract, silver nitrate concentration 0.5 mmol/L, incubation temperature at 80 ℃ and duration time was 15 min, the diameter of silver nanoparticles was 7 （±23%） nm.When the concentration of silver nanoparticles was at 0.30 μg/ml, there were antibacterial activity in both Mycobacterium avium and H37Rv.

关 键 词：银化合物 金属纳米粒子 微生物敏感性试验 分枝杆菌 鸟 剂量效应关系 药物 

分 类 号：R378.91[医药卫生—病原生物学]