MicroRNA-34c regulates porcine granulosa cell function by targeting forkhead box O3a  

作　　者：XU Yuan ZHANG Ai-ling ZHANG Zhe YUAN Xiao-long CHEN Zan-mou ZHANG Hao LI Jia-qi 

机构地区：[1]Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding,College of Animal Science,South China Agricultural University [2]College of Biological and Food Engineering,Guangdong University of Education

出　　处：《Journal of Integrative Agriculture》2017年第9期2019-2028,共10页农业科学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (31201771);the earmarked fund for the China Agriculture Research System (CARS-36)

摘　　要：Granulosa cells（GCs） are somatic cells of ovary, the behaviors of GCs are important for ovarian function. MicroRNAs（miRNAs） are a class of endogenous 18–24 nucleotide（nt） non-coding RNAs, some of which have been shown to be important regulators of GCs function. miR-34c involved in the regulation of various biological processes and was identified to be a pro-apoptotic and anti-proliferative factor in many cell types. However, the roles of miR-34c in GCs function remain unknown. In this study, we used Annexin V-FITC and Ed U assays to demonstrate that miR-34c exerted pro-apoptotic and anti-proliferative effects in porcine GCs. Dual-luciferase reporter assays, quantitative real-time PCR（q RT-PCR） and Western blotting identified Forkhead box O3a（Fox O3a） as a direct target gene of miR-34c. The overexpression of FoxO3a rescued the phenotypic change caused by miR-34c in porcine GCs. In conclusion, miR-34c regulate the function of porcine GCs by targeting FoxO3a.Granulosa cells（GCs） are somatic cells of ovary, the behaviors of GCs are important for ovarian function. MicroRNAs（miRNAs） are a class of endogenous 18–24 nucleotide（nt） non-coding RNAs, some of which have been shown to be important regulators of GCs function. miR-34c involved in the regulation of various biological processes and was identified to be a pro-apoptotic and anti-proliferative factor in many cell types. However, the roles of miR-34c in GCs function remain unknown. In this study, we used Annexin V-FITC and Ed U assays to demonstrate that miR-34c exerted pro-apoptotic and anti-proliferative effects in porcine GCs. Dual-luciferase reporter assays, quantitative real-time PCR（q RT-PCR） and Western blotting identified Forkhead box O3a（Fox O3a） as a direct target gene of miR-34c. The overexpression of FoxO3a rescued the phenotypic change caused by miR-34c in porcine GCs. In conclusion, miR-34c regulate the function of porcine GCs by targeting FoxO3a.

关 键 词：porcine microRNA-34c FoxO3a granulosa cell function 

分 类 号：S828[农业科学—畜牧学]