吡咯喹啉醌对衰老大鼠学习记忆的影响及机制研究  

Effect of pyrroloquinoline quinone on learning and memory ability of apolexis rats

作　　者：唐靓[1] 李林子[1] 胡文敏[1] 胡志航[1] 张岭[1] 张丽婧[1] 梅松[1] 陈建国[1] 刘冬英[1] 刘臻[1] 王茵[1] 

机构地区：[1]浙江省医学科学院,浙江杭州310013

出　　处：《中国食品卫生杂志》2017年第4期407-411,共5页Chinese Journal of Food Hygiene

基　　金：浙江省营养学医学支撑学科建设(11-zc03);浙江省科技厅项目(2017F30001;2015C33109);浙江省医药卫生科技计划(2014ZDA004;2016KYB071;2017KY299);浙江省自然基金项目(LY17H280008);食品(保健食品)质量安全评价研究实验室建设(2016F10002)

摘　　要：目的研究吡咯喹啉醌(pyrroloquinoline quinone,PQQ)对受到氧化损伤的神经细胞的修复作用,探讨PQQ在因衰老产生机体氧化损伤的大鼠体内、脑内的抗氧化作用,以及该抗氧化作用对衰老大鼠学习记忆能力产生的影响。方法使用H_2O_2诱导PC12神经细胞(大鼠肾上腺髓质嗜铬瘤分化细胞株)氧化损伤,随后用噻唑兰法检测PQQ对PC12的修复作用。用PQQ(0、10、20、40 mg/kg)灌胃18月龄雄性SD大鼠,4周后用Morris水迷宫试验测定大鼠的学习记忆能力,6周后测定血清和脑组织的氧化损伤水平和抗氧化能力。结果 200 nmol/L的PQQ使PC12细胞的存活率从59.1%增加到90.5%;与衰老模型组比较,PQQ中和高剂量组(20、40 mg/kg)大鼠在Morris水迷宫试验中的5 d潜伏期缩短、5 d游泳总路程减少,PQQ各剂量组(10、20、40 mg/kg)7 d穿越次数增加、7 d第一次平台穿越时间减少。同时PQQ各剂量组大鼠血清和脑组织中丙二醛、脑组织中脂褐素水平降低,中和高剂量组(20、40 mg/kg)血清和脑组织中超氧化物歧化酶水平、谷胱甘肽过氧化物酶活力以及脑组织中抗氧化能力增强。结论 PQQ可修复神经细胞的氧化损伤,证实了PQQ能够在体内与大脑中同样发挥抗氧化作用,增加衰老大鼠学习记忆能力。Objective This study aimed to investigate the role of pyrroloquinoline quinone（ PQQ） in repairing oxidative nerve cells,and to study the antioxidant capacity of PQQ on the oxidative damage of rats caused by apolexis,as well as the effects on learning and memory abilities of apolexis rats. Methods Oxidative damage of PC12 was induced by H2O2,and the repairing rate of PQQ on oxidative PC12 cells was tested by methylthiazolyldiphenyl-tetrazolium bromide assay kit. The18-month-old male SD rats were administered PQQ（ 0,10,20,40 mg/kg）. After 4 weeks,Morris water maze test was used to test the learning and memory ability. After 6 weeks,serum and brain tissue related indicators and antioxidant capacity were recorded. Results The survival rate of PC12 cells increased from 59. 1% to 90. 5% with 200 nmol/L PQQ.Compared with the apolexis model group,the latency of the PQQ group（ 20,40 mg/kg） was shortened in the Morris water maze experiment,the swimming distance was reduced,pass-through counts were increased,and the first secure platform pass-through was reduced. Meanwhile,the levels of malondialdehyde and lipofuscin in serum and brain tissue of PQQ group decreased,the levels of superoxide dismutase,glutathione peroxidase vitality,antioxidant capacity of PQQ group（ 20,40mg/kg） were enhanced. Conclusion PQQ could repair the oxidative damage of nerve cells,and it was confirmed that PQQ could play the same antioxidant effect in body and brain,and increase the learning and memory ability of apolexis rats.

关 键 词：吡咯喹啉醌 PC12细胞 衰老 抗氧化 学习记忆 衰老模型 大鼠 水迷宫 

分 类 号：R965[医药卫生—药理学]