二苯基氯化碘盐与电压门控质子通道Hv1的C-端结构域的相互作用(英文)  

Interaction of Diphenyleneiodonium with the Carboxyl-terminal Domain of Voltage-gated Proton Channel Hv1

作　　者：张尚荣 赵青[1] 李树杰[1] 

机构地区：[1]南开大学物理科学学院生物物理系,天津300071

出　　处：《南开大学学报（自然科学版）》2017年第4期63-69,共7页Acta Scientiarum Naturalium Universitatis Nankaiensis

基　　金：Supported by the China Postdoctoral Science Foundation Funded Project(2015M581289)

摘　　要：电压门控质子通道(Hv1)在"呼吸爆发"过程中参与细胞内p H的调节,以补偿NADPH氧化酶产生电子时的电荷.Hv1胞内C端结构域通过α螺旋二级结构形成二聚体,这种结构对蛋白质定位和在吞噬体中可能感应氧化还原反应起重要作用.二苯基氯化碘盐(DPI)经常被用来抑制细胞内活性氧的产生.通过荧光光谱实验研究了DPI与Hv1的C端结构域之间的相互作用.静态荧光猝灭的存在表明DPI与Hv1的C端结构域之间有相互作用.此外,锌离子的存在使DPI与Hv1的C端结构域之间的相互作用增强,表明锌离子影响DPI与Hv1的C端结构域之间的相互作用.研究结果表明,DPI与Hv1的C端结构域之间有相互作用,这可能为DPI抑制细胞内活性氧的产生机理提供新的解释.The voltage-gated proton channel Hv1 is involved in regulating intracellular p H during respiratory burst,in which it compensates electrically for the electrogenic activity of NADPH oxidase（NOX）.The intracellular C-terminal domain of Hv1 forms a dimer via a parallelα-helical coiled-coil,which is essential for the protein localization and may play a role in redox sensing in the phagosome.Diphenyleneiodonium（DPI）has frequently been used to inhibit intracellular reactive oxygen species （ROS）production.Herein,we investigated the interaction of DPI with the carboxyl-terminal domain of voltage-gated proton channel Hv1 in absence or presence of Zn2＋ ion by fluorescence spectroscopy in vitro.The static fluorescence quenching indicates the existence of strong binding between DPI and the carboxyl-terminal domain of Hv1.Moreover,the interaction of DPI with the carboxyl-terminal domain of Hv1 is stronger in presence of Zn2＋ ion,which implicates that Zn2＋ ion has an effect on the interaction of DPI with the carboxyl-terminal domain of Hv1.Our results suggested that DPI interacts with the C-terminal domain of Hv1,which may shed light on a new probable explain for how DPI inhibit the generation of intracellular ROS.

关 键 词：二苯基氯化碘盐 电压门控质子通道Hv1 C端结构域 相互作用 

分 类 号：Q51[生物学—生物化学]