苦碟子注射液对氧糖剥夺损伤的C6胶质细胞凋亡与自噬调节作用  

Effect of Kudiezi Injection between Autophagy and Apoptosis in C6 Glial Cells Exposed by Oxygen Glucose Deprivation

作　　者：郑宏[1] 张昕洋 鲁雨荍 曾子修 梁晓[2] 刘雪梅[1] 

机构地区：[1]北京中医药大学东方医院,北京100078 [2]北京中医药大学,北京100029

出　　处：《中国中医基础医学杂志》2017年第8期1154-1157,共4页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：国家国际科技合作专项项目(2015DFA31130)-清热活血组分治疗急性脑梗死;国家重点基础研究发展计划项目(973项目)(2012CB518406)-治疗心血管疾病有效方剂组分配伍规律研究;国家自然科学基金资助项目(81202683)-从胶质细胞TAK1-NF-kB通络调控炎症反应失衡切入研究清脑丸对急性脑缺血的干预效应机制

摘　　要：目的:研究苦碟子注射液对氧糖剥夺损伤C6胶质细胞凋亡与自噬的调节作用,明确其对氧糖剥夺损伤胶质细胞的保护作用。方法:采用氧糖剥夺/复氧(OGD/R)的方法建立C6胶质细胞损伤模型,按随机数字表法分为空白对照组、氧糖剥夺/复氧模型组、苦碟子注射液组、自噬抑制剂(3-MA)组4组,Annexin V FITC/PI双染流式细胞仪检测细胞凋亡率,免疫荧光染色法观察活化caspase-3表达,western blotting法检测LC3Ⅱ蛋白表达。结果:氧糖剥夺/复氧损伤后LC3Ⅱ蛋白表达增加,细胞凋亡率升高,苦碟子注射液能够显著降低C6胶质细胞凋亡率,增加LC3Ⅱ蛋白高表达。结论:苦碟子注射液能够降低C6胶质细胞凋亡率,进一步激活细胞自噬而发挥保护作用。Objectives： To study the regulatory effects of Kudiezi injection on the apoptosis and autophagy of C6 glial cells,which are exposed oxygen glucose deprivation/reoxygenation injury,and clarify its neuroprotective effect on glial cells damaged by oxygen and glucose deprivation. Methods： C6 glia cells were exposed to oxygen-glucose deprivation（ OGD/R） stress. The cells were randomly divided into four groups： control group,OGD/R group,Kudiezi injection group and autophagy inhibit（ 3-MA） group. Apoptosis and cleaved caspase-3 were detected by Annexin V FITC/PI double staining and immunofluorescence staining. The protein expression of LCⅡ3 was detected by western blotting. Results：Oxygen-glucose deprivation/reoxygenation injury C6 cells activated LCⅡ3 protein expression increased,apoptosis increased significantly. Kudiezi injection could further activate autophagy and increase the protein expression of LCⅡ3,while reduce the rate of apoptosis. Conclusons： Kudiezi injection can reduce the apoptosis,and further activate autophagy,and plays a protective role in C6 glia cells.

关 键 词：C6胶质细胞 氧糖剥夺 急性脑梗死 凋亡 自噬 

分 类 号：R285.5[医药卫生—中药学]