金华地区慢性乙型肝炎患者HBV基因型及耐药基因位点突变类型分析  

Genotypes and mutation patterns of HBV gene in chronic hepatitis B patients in Jinhua

作　　者：刘颖[1] 朱以军[1] 王月[1] 

机构地区：[1]金华市中心医院中心实验室,321000

出　　处：《浙江医学》2017年第14期1158-1161,共4页Zhejiang Medical Journal

摘　　要：目的分析金华地区慢性乙型肝炎患者HBV基因型分布特征及HBV耐药基因位点突变情况,并探讨两者的关系。方法选取104例经核苷(酸)类似物治疗的慢性乙型肝炎患者为研究对象,采用Sanger双脱氧法测序技术检测患者血清HBV的基因型和10种常见的HBV P区耐药基因位点突变情况,并分析两者关系。结果本组慢性乙型肝炎患者中,HBV B基因型占46.15%,C基因型占53.85%,未发现其他基因型。检测出发生耐药基因位点突变61例,突变率为58.65%,其中C基因型占65.57%,B基因型占34.43%。HBV P区耐药基因位点突变率最高的为M204V/I(73.85%),其次为L180M(36.92%)和A181V/T(23.08%)。HBV P区耐药基因发生单位点突变的占50.82%,其中M204I突变类型最多(29.51%);发生多位点突变的占49.18%;以M204I和M204V/I+L180M两种突变类型最常见。HBV C基因型的患者耐药基因突变率高于B基因型的患者(71.43%vs 43.75%,P<0.05),其中C基因型的患者A181V/T位点突变率高于B基因型的患者(14.29%vs 2.08%,P<0.05)。结论金华地区慢性乙型肝炎患者HBV基因型以B、C基因型为主;HBV P区耐药基因位点突变类型以M204I和M204V/I+L180M两种类型最常见;HBV C基因型的患者比B基因型的患者更易发生耐药突变,且更易发生A181V/T位点突变。rolled in the study.Sanger sequence analysis of HBV genotypes and 10 common sites of HBV P region were performed.Results There were 46.15% cases of B genotype and 53.85% of C genotype among 104 chronic hepatitis B patients.Other genotypes were no detected.HBV P gene mutation were detected in 61 cases （58.65%）;there was 65.57% in HBV C genotype and 34.43% in B genotype.M204V/I was the most common site of HBV P gene mutation （73.85%）,followed by L180M （36.92%） and A181V/T （23.08%）.50.82% cases had single site mutation and the most mutation pattern was M204I （29.51%）;and 49.18% had multiple sites mutation;the most common mutation patterns were M204I and M204V/I＋L180M.The resistance mutation rate in C genotype was higher than that in B genotype（71.43% vs 43.75%,P〈0.05） and the mutation rate of A181V/T in C genotype cases was higher than that in B genotype cases （14.29% vs 2.08%,P〈0.05）.Conclusion Genotype B and C are the main genotypes of HBV in this study.The most common mutation patterns of HBV P gene were M204I and M204V/I＋L180M.Genotype C patients had more mutations than genotype B and the A181V/T mutation pattern was more common in genotype C.

关 键 词：慢性乙型肝炎 耐药突变 基因型 核苷(酸)类似物 

分 类 号：R512.62[医药卫生—内科学]