不同茭白品种中菰黑粉菌的鉴定及ISSR多态性分析  

Identification and ISSR polymorphism analysis of Ustilago esculenta isolated from Zizania latifolia

作　　者：康璐瑶 崔海峰 张雅芬 张子杰 俞晓平 叶子弘 

机构地区：[1]中国计量大学生命科学学院浙江省生物计量与检验检疫技术重点实验室,浙江杭州310018

出　　处：《菌物学报》2017年第9期1210-1221,共12页Mycosystema

基　　金：国家自然科学基金(3147085);国家科技支撑计划项目子课题(2012BAD27B01‐3)~~

摘　　要：菰黑粉菌是茭白的内生真菌,对茭白孕茭具有重要作用。本文分离获得了13个不同茭白品种和孕茭表型的菰黑粉菌菌株(M‐T型和T型),ITS序列鉴定分析发现:13个菌株都属于菰黑粉菌,它们的ITS序列仅在ITS1序列区间存在3个碱基位点的变异;基于ISSR技术比较分析了菰黑粉菌菌株间的遗传多态性,聚类分析表明,菰黑粉菌菌株的遗传多态性与茭白栽培特点及孕茭表型密切相关,本研究有助于茭白种质资源的鉴定及保护,并为茭白田间栽培管理提供了技术支持和理论依据。As an endogenous fungus of Zizania latifolia, Ustilago esculenta plays an important role in the formation of the swollen stem. In this study, 13 strains of U. esculenta （M-T type and T type） were isolated from different varieties and phenotypes of Z. latiJ：olia. According to the ITS sequences and comparative analysis, all of the 13 strains was identified as U. esculenta. There were three bases＇ variations in ITS1 sequence among different strains. Based on ISSR, genetic polymorphism analysis of the 13 strains was carried out. Cluster analysis showed that genetic polymorphism of U. esculenta strains are closely related to the cultivation characteristics and phenotypes of Z. latifolia. Genetic polymorphism of U. esculenta could provide complementary information in identifying and protecting germplasm resources of Z. latifolia.

关 键 词：茭白 菰黑粉菌 内转录间隔区序列 简单重复序列间扩增分析 

分 类 号：S645.2[农业科学—蔬菜学]