耐旱基因cspB转化大豆的研究  

Genetic Transformation of Soybean with Drought Tolerance Gene cspB from Bacillus subtilis

作　　者：孙雪慧[1] 蔡勤安[2] 尚丽霞[2] 马瑞[2] 杨向东[2] 于志晶[2] 魏建[1] 

机构地区：[1]长春师范大学,吉林长春130032 [2]吉林省农业科学院生物技术研究所/吉林省农业生物技术重点实验室,吉林长春130033

出　　处：《大豆科学》2017年第5期699-704,共6页Soybean Science

基　　金：转基因生物新品种培育重大专项(20148X0800403B-002);吉林省科技厅重点科技攻关项目(20130206005NY)

摘　　要：为提高大豆的耐旱性,本研究根据大豆偏爱的密码子将来源于枯草杆菌抗旱相关基因cspB序列进行了优化,并构建了植物表达载体pCAMBIA3301-cspB,利用农杆菌介导法将cspB基因导入大豆栽培品种Bert中。用于遗传转化子叶节外植体共3 800个,经PCR和Southern鉴定,并结合PPT抗性筛选,共获得了95棵阳性转基因植株,转化率为2.5%。经初步筛选,获得了7份耐旱性较好的转基因大豆新材料。这些材料将进一步用于耐旱转基因大豆新品种培育工作。To improve the drought tolerance of soybean, a vector pCAMBIA3301-cspB with drought tolerance related modified gene cspB, was constructed. The modified cspB gene was introducted into soybean variety Bert by Agrobacterium-mediated transformation. In this experiment total 3 800 cotyledonary node explants were infected and 95 independent transgenic plants were obtained by PCR and Southern blot analysis combined with PPT selection. The transformation efficiency was 2. 5%. Sev- en transgenic plants with drought tolerance were selected,which could be used for soybean drought tolerance breeding in fur- ther.

关 键 词：大豆 耐旱 cspB基因 遗传转化 

分 类 号：S565.1[农业科学—作物学]