轴突导向因子-1对糖尿病大鼠视网膜Müller细胞活化的影响  

The effect of netrin-1 on activation of Müller cell in diabetes rats

作　　者：周贤慧 孟旭霞[1] 胡迭 

机构地区：[1]青岛大学附属医院眼科,266500 [2]山东省烟台业达医院眼科 [3]青岛眼科医院

出　　处：《中华眼底病杂志》2017年第5期527-529,共3页Chinese Journal of Ocular Fundus Diseases

基　　金：山东省自然科学基金（ZR2010HM062）感谢烟台市业达医院眼科姜文科、刘晓玲医师对文稿撰写提供的帮助

摘　　要：目的观察外源性轴突导向因子-1（netrin-1）对糖尿病（DM）大鼠视网膜Müller细胞活化的影响。方法健康清洁级雄性Sprague—Dawley大鼠50只，采用随机数字表法随机分为正常对照组（A组）、正常＋平衡盐溶液（BSS）组（B组）、正常＋netrin-1 100μg／ml组（C组）、DM＋BSS组（D组）、DM＋netrin-1100μg／ml组（E组），每组10只。D、E组大鼠按60mg／kg的剂量，左下腹腔注射链脲佐菌素诱导DM动物模型。采用免疫组织化学染色法检测大鼠视网膜Müller细胞标志物胶质原纤维酸性蛋白（GFAP）的阳性表达；荧光定量聚合酶链反应检测大鼠视网膜GFAPmRNA的表达。结果免疫组织化学染色结果显示，A～C组大鼠视网膜仅在神经纤维层及神经节细胞层可见少量GFAP阳性表达。D组大鼠视网膜GFAPg~性表达增多。E组大鼠视网膜GFAP阳性表达较D组减少。A～E组大鼠视网膜GFAP阳性表达、mRNA表达比较，差异有统计学意义（F=203．43、72．91，P=0．00、0．00）。与A组比较，D组大鼠视网膜GFAP阳性表达、mRNA表达明显增多，差异有统计学意义（t=-26．01、22．26，P=0．00、0．00）。E组大鼠视网膜GFAP阳性表达、mRNA表达较D组明显下降（t=10．78、3．93，P=0．00、0．00），但仍高于A组（t=7．00、-9．82，P=-0．00、0．00），差异均有统计学意义。A、C组大鼠视网膜GFAP阳性表达、mRNA表达比较，差异均无统计学意义（t=-0．29、0．50，P=-0．77、0．62）。结论Netrin-1可降低DM大鼠视网膜Müller~胞活化，减少视网膜GFAP表达。Objective To observe the effect of netrin-1 on retinal MOiler cells in diabetes mellitus （DM） rats. Methods Fifty Sprague-Dawley rats were randomly divided into the normal control group （group A）, normal ＋ balanced salt solution （BSS） group （group B）, normal＋netrin-1 group （group C）, DM＋BSS group （group D） and DM＋netrin-1 group （group E）, with 10 rats in each group. DM rats were induced by intraperitoneal injection of Streptozotocin （60 mg/kg）. The expression level of glial fibrillary acidic protein （GFAP） on retinal MOiler cells was determined by immunohistochemistry, the level of GFAP mRNA was analyzed by real-time fluorescence quantitative reverse transcription polymerase chain reaction. Results Immunohistochemistry showed that GFAP was distributed in retinal ganglion cells and retinal nerve fiber layer in group A, B and C. Compared to group B, GFAP staining was brighter in the group D. There were significant differences in the expression of GFAP protein and mRNA among groups A-E （F=203.43, 72.91; P=-0.00, 0.00）, they were higher in group D than group A （t=-26.01, 22.26; P=0.00, 0.00）, and group E （t=-10.78, 3.93; P=0.00, 0.00）. They were higher in group E than group A （t=7.00, -9.82; P=0.00, 0.00）. There were no significant differences in between group A and group C （t=-0.29, 0.50; P=0.77, 0.62）. Conclusion The expression of GFAP in MOiler cells of DM rats could be decreased by injecting netrinol into vitreous.

关 键 词：糖尿病视网膜病变/病理生理学 小神经胶质细胞/生理学 神经生长因子类 动物实验 

分 类 号：R587.2[医药卫生—内分泌] R774.1[医药卫生—内科学]