丹参酮ⅡA对放射性内耳损伤的保护作用  

Protective effect of tanshinone ⅡA on radiation injury of cochlea

作　　者：谭佩欣[1] 杜莎莎[2] 任陈[2] 

机构地区：[1]广东省人民医院放疗科,广州市510080 [2]南方医科大学南方医院,广州市510515

出　　处：《实用医学杂志》2017年第17期2821-2826,共6页The Journal of Practical Medicine

基　　金：国家自然科学基金项目(编号:81673103);广东省自然科学基金项目(编号:2015A030313262)

摘　　要：目的探索丹参酮ⅡA注射液对豚鼠放射性内耳损伤的保护作用。方法 20只豚鼠(40耳)随机分为对照组(Control组)、单纯照射组(RT组)、照射及药物处理组(RT+D组)及单纯药物组(D组),在放射线照射前2 d开始对RT+D组及D组豚鼠进行丹参酮ⅡA处理,连续腹腔注射7 d,而对Con-trol组及RT组豚鼠在相应时间点腹腔注射等量生理盐水。分别在放射线照射前后不同时间点[照射前或丹参酮处理前(d0)、照射完毕第7天(d7)以及第8周(d8w)]进行畸变产物耳声发射技术(DPOAE)及听性脑干反应(ABR)等听力学检测,并在最后一次听力学检测完毕后处死实验动物并进行耳蜗组织学检查。结果放射线照射后RT组(F=1 134.064,P<0.001)及RT+D组(F=664.185,P<0.001)DPOAE幅值均有显著下降,但RT+D组DPOAE幅值下降幅度较RT组小(P<0.001),RT组(F=12.228,P=0.002<0.05)豚鼠ABR阈值有显著上升,而RT+D组(F=2.867,P=0.102>0.05)豚鼠ABR阈值在放射线照射前后差异无统计学意义。组织学检查发现RT+D组豚鼠Corti器毛细胞、血管纹及螺旋神经节细胞损害程度较RT组轻。结论丹参酮ⅡA注射液对豚鼠放射性内耳损伤具有一定保护作用。Objective To explore the protective effects on radiation injury of guinea pigs＇ cochlea by sulfotanshinone ⅡA sodium injection. Methods 20 guinea pigs （40 ears） were randomly divided into control group, radiotherapy （RT） group, radiotherapy and drug （RT ＋ D） group and drug （D） group. The guinea pigs in the RT ＋ D group and the D group were injected intraperitoneally with tanshinone on 2 days before the irradiation and lasted for 7 days, while the guinea pigs in the control group and the RT group were injected intrapertoneally with equal amount of physiological saline at the same time. All the guinea pigs underwent audiologic test with DPOAE and ABR at different time points before and after the irradiation, which were d0, d7 and d8w. All pigs were sacrificed after the completion of the audiologic experimental and for histologic observation. Results After the irradiation, the DPOAE amplitudes of the guinea pigs in both RT group （F= 1 134.064, P 〈 0.001 ） and RT ＋ D group （F= 664.185, P 〈 0.001 ） decreased significantly, but the range in the RT ＋ D group was lower than in the RT group （P 〈 0.001 ）. The ABR threshold of the guinea pigs raised significantly in the RT group （F= 12.228, P= 0.002 〈 0.05）, but not in the RT ＋ D group （F=2.867 ,P=0.102 〉 0.05）. Histological examination showed that the damage degree of hair cells, stria vascularis and the spiral ganglion cells in the organ of Corti of the guinea pigs were lower than in the RT ＋ D group, but higher in the RT group. Conclusion Sulfotanshinone ⅡA sodium injection can provide protection against radiation injury of guinea cochlea.

关 键 词：放射损伤 耳毒性 听性脑干反应 畸变产物发射试验 丹参酮 

分 类 号：R764[医药卫生—耳鼻咽喉科]