呋喃甲醛缩对硝基苯胺与汞离子的荧光猝灭反应及其应用  

Fluorescence Quenching Reaction of Furfural p-Nitroaniline with Mercury Ion and Its Application

作　　者：何立坚 张锐明 章汝平[2] 

机构地区：[1]厦门华厦学院,厦门361024 [2]龙岩学院,龙岩364012

出　　处：《理化检验（化学分册）》2017年第9期993-996,共4页Physical Testing and Chemical Analysis(Part B:Chemical Analysis)

基　　金：国家自然科学基金青年基金资助项目(21607069);福建省教育厅中青年教师教育科研项目(JA15507);龙岩市科技局重点计划项目(2015LY25)

摘　　要：合成了呋喃甲醛缩对硝基苯胺(FFNA)荧光试剂,在pH 9.06的四硼酸钠缓冲溶液中,该试剂与汞离子以1∶1的物质的量比形成络合物,使得FFNA的荧光猝灭,据此建立了测定汞离子的荧光光谱法。激发波长和发射波长分别为235,330nm时,体系的荧光强度改变(ΔI_F)与汞离子的浓度在4.0×10^(-8)~1.1×10^(-7) mol·L^(-1)内呈线性关系,检出限(3s/k)为2.24×10^(-8) mol·L^(-1)。该方法用于化妆品中微量汞的测定,结果与原子吸收光谱法相一致,加标回收率在99.0%~99.5%之间,测定值的相对标准偏差(n=6)在3.1%~3.5%之间。A fluorescent reagent of furfural p-nitroaniline （FFNA） was synthesized.In a Na2B4O7 buffer solution of pH 9.06,FFNA reacted with Hg^2＋ to form a complex with the amount-of-substance ratio of 1∶1,resulting in fluorescence quenching of FFNA.Based on this fact,a mehtod of fluorescence spectroscopy was developed for determination of Hg^2＋.At the excitation wavelength of 235 nm and emission wavelength of 330 nm,linear relationship was found between the change of the fluorescence intensity with the concentration of Hg^2＋ in the range of 4.0×10^-8-1.1×10^-7 mol·L^-1,with detection limit （3s/k） of 2.24×10^-8 mol·L^-1.The proposed method was used for the determination of trace mercury in cosmetics samples,giving results in agreement with those obtained by atomic absorption spectrophotometry.Recovery rates obtained by standard addition method were in the range of 99.0%-99.5%,and RSDs （n=6） were in the range of 3.1%-3.5%.

关 键 词：呋喃甲醛缩对硝基苯胺 汞离子 荧光猝灭 荧光光谱法 

分 类 号：O657.3[理学—分析化学]