外源K-IBA对北美香柏扦插生根和内源激素含量变化的影响  

Effects of K-IBA treatments on adventitious rooting and endogenous hormones contents of shoot cuttings of Thuja occidentals L.

作　　者：何崇单 姚锐[2] 黄瑞春 米小琴 张超[2] 谭益民[1] 王义强[1,2] 

机构地区：[1]中南林业科技大学经济林培育与保护教育部重点实验室,湖南吉首416713 [2]中南林业科技大学中南林业科技大学生物技术实验室,湖南长沙410004 [3]湘西州森林生态研究实验站,湖南吉首416713

出　　处：《中南林业科技大学学报》2017年第10期7-12,共6页Journal of Central South University of Forestry & Technology

基　　金：国家林业局"948"项目(2013-4-25)

摘　　要：以北美香柏Thuja occidentals L.2年生枝条为材料,采用3-吲哚丁酸钾盐(K-IBA)3个浓度(3 000、6 000、10 000 mg/L)处理扦插生根试验,清水处理为对照。扦插60天后统计插穗生根率、平均根长、最长根长、平均生根数。扦插后每隔15 d从6 000 mg/L K-IBA与对照处理插穗的基部取样,测定生根过程中内源激素吲哚乙酸(IAA)、赤霉素(GA)、脱落酸(ABA)和玉米素(ZT)含量的变化。结果表明:外源K-IBA对北美香柏扦插生根有促进作用,其中6 000 mg/L K-IBA的处理效果最好,其生根率为87.78%、生根数22.33根、平均根长12.33 mm、最长根长75.20 mm,显著高于对照。与对照相比,6 000 mg/L K-IBA处理组的内源IAA含量、GA含量提高,而内源ABA、ZT含量下降,促使生根率提高。在北美香柏扦插生根过程中,K-IBA处理调节了内源激素IAA、GA、ABA和ZT含量的变化,而内源激素的动态变化,共同调控插穗不定根的形成。The shoot cuttings of Thuja occidentals L. were collected and treated with indole-3-butyric acid （K-IBA） （concentrations 3 000, 6 000 and 10 000 mg/L）, Water treatment as a control. The rooting rate, average root length, the longest root length and average rooting number were recorded after cutting for 60 days; The endogenous hormones（IAA, GA,ABA, ZT） contents of cuttings of 6 000 mg/L K-IBA and control treatment were detected every 15 days interval.The significant difference was found among the four treatments. K-IBA promoted the rooting of Thuja occidentals L, Among them, 6 000 mg/L K-IBA has the best treatment effect, which was significantly higher than that of CK. The rooting rate was 87.78%, the number of rooting was 22.33, the average root length was 12.33 mm, the longest root length was 75.20 mm. Compared with the control, the content of endogenous IAA and GA increased in 6 000 mg/L K-IBA treatment group, while the contents of endogenous ABA and ZT decreased, then promoted the rooting. K-IBA regulates the changes of endogenous hormones IAA, GA, ABA and ZT, and the dynamic changes of endogenous hormones regulate the formation of adventitious roots in the process of rooting.

关 键 词：北美香柏 扦插 不定根发育 内源激素 

分 类 号：S791.41[农业科学—林木遗传育种]