安尔碘皮肤消毒剂对临床耐药菌株最小杀菌浓度测定  

Detection of minimal bactericidal concentration of Anerdian skin disinfectant against clinical drug-resistant bacterial strains

作　　者：文细毛[1] 黄勋[1] 李洁[1] 黄昕[1] 吴安华[1] 

机构地区：[1]中南大学湘雅医院,湖南长沙410078

出　　处：《中国消毒学杂志》2017年第8期712-714,共3页Chinese Journal of Disinfection

摘　　要：目的探讨含碘消毒剂对临床耐药菌株最小杀菌浓度(MBC)。方法以安尔碘为研究对象,采用对倍稀释法,以不同浓度对临床耐药菌株和标准菌株大肠埃希菌(8099)和金黄色葡萄球菌(ATCC 6538)分别作用1、3、5 min,观察杀灭效果。结果安尔碘的有效碘含量为7.81 mg/L,作用1 min可将所有检测菌全部杀灭;有效碘含量为3.90 mg/L,作用5 min可将检测的所有菌株全部杀灭。但作用1 min,革兰阳性菌中有7株临床分离的金黄色葡萄球菌的MBC(7.81 mg/L)高于其标准菌株(3.90 mg/L);作用5 min,革兰阴性菌中有18株菌的MBC(3.90mg/L)高于标准菌株大肠埃希菌(<1.95 mg/L)。结论绝大部分临床多重耐药菌对安尔碘的抗力未超过标准菌株,仍呈现出良好的杀灭效果。Objective To determine the minimal bactericidal concentration（ MBC） of iodine-containing disinfectant against clinical drug-resistant bacterial strains. Methods Anerdian was used as studied object,doubling dilution method was adopted,Anerdian of different concentrations was contacted with clinical drug-resistant bacterial strains,standard strain Escherichia coli（ 8099）,and Staphylococcus aureus（ ATCC 6538） for 1,3,and 5 min respectively,and bactericidal efficacy was observed. Results All detected bacteria were killed by Anerdian which containing 7. 81 mg/L available iodine in1 min,by Anerdian which containing 3. 90 mg/L available iodine in 5 min. But when contact time was 1min,MBCs of 7strains of clinically isolated Staphylococcus aureus（ 7. 81 mg/L） were higher than that of standard strain of Staphylococcus aureus（ 3. 90 mg/L）; when contact time was 5 min,MBCs of 18 strains of isolated Gram-negative bacteria（ 3. 90 mg/L）were higher than standard strain of Escherichia coli（ 〈1.95 mg/L）. Conclusion Resistance of most clinical multidrugresistant strains to Anerdian is not higher than the standard strain,and Anerdian still presents a good bactericidal efficacy.

关 键 词：有效碘 临床耐药菌株 最小杀菌浓度 消毒剂 

分 类 号：R187[医药卫生—流行病学]