红景天苷对慢性阻塞性肺疾病小鼠保护作用机制研究  

Protective Effects of Salidroside on Chronic Obstructive Pulmonary Disease in Mice

作　　者：姚立军[1] 刘媛[1] 

机构地区：[1]苏州大学附属第一医院药学部,江苏苏州215006

出　　处：《中国药学杂志》2017年第17期1515-1518,共4页Chinese Pharmaceutical Journal

摘　　要：目的研究红景天苷对慢性阻塞性肺疾病(COPD)小鼠肺损伤的保护作用及其机制研究。方法采用反复香烟烟熏法复制COPD小鼠模型。观察各组肺组织的病理学改变,测定血清白细胞介素(IL)-6、IL-1β、肿瘤坏死因子(TNF)-α和肺组织中丙二醛(MDA)、超氧化物歧化酶(SOD)的含量。Western blot检测肺组织中HO-1、Nrf-2、NF-κBp65、p-NF-κBp65、IκBα、pIκBα等氧化和炎症蛋白的表达。结果红景天苷治疗组中肺损伤明显减轻;显著降低血清中IL-6、IL-1β和TNF-α含量;增加肺组织中SOD、降低MDA含量;减少p-IκBα、p-NF-κBp65表达,增加Nrf-2、HO-1表达。结论红景天苷对慢性阻塞性肺疾病小鼠肺损伤具有保护作用,其作用可能与其调节氧化应激诱导的炎症反应相关。OBJECTIVE To evaluate the effects of salidroside on lung injury in chronic obstnxctive pulmonary disease （COPD） mice. METHODS Smoked cigarettes （CS） method was used to establish the COPD model in mice. Investigate the morphological changes. IL-6, IL-1β, TNF-a, SOD and MDA were measured. Western blot was applied to evaluate the related proteins in lung, such as p-IKBa, Nrf-2, HO-1, p-NF-KBp65. RESULTS H＆E staining demonstrated that salidroside inhibited CS-induced pathological injury in lung tissue, decreased cytokines. SOD was increased and MDA was decreased. Salidroside also increased Nrf-2, HO-1 and decreased p-IKBa, p-NF-KBp65. CONCLUSION Salidroside possesses protective effect on lung injury in COPD mice, the regula- tion of oxidative stress and inflammation may be the mechanism.

关 键 词：红景天苷 慢性阻塞性肺疾病 氧化应激 炎症 

分 类 号：R965[医药卫生—药理学]