反义抑制miR-222表达对替莫唑胺所致神经胶质瘤细胞DNA损伤作用的影响  

Effect of antisense inhibition of miR-222 expression on DNA damage induced by temozolomide in glioma cells

作　　者：郭国强 黄浩宇 朱志良[3] 袁建辉[4] 胡大林[2] 张继平 

机构地区：[1]深圳市宝安区西乡预防保健所,深圳广东518100 [2]南方医科大学公共卫生学院 [3]深圳市宝安区疾病预防控制中心 [4]深圳市疾病预防控制中心 [5]南方医科大学附属佛山医院

出　　处：《毒理学杂志》2017年第4期273-276,共4页Journal of Toxicology

基　　金：广东省科技计划项目(2014A020212206);深圳市科技研发资金(JCYJ20150331102453903)

摘　　要：目的探讨miR-222低表达的神经胶质瘤细胞(T98G-miR-222-)对替莫唑胺(TMZ)的DNA损伤反应。方法运用脂质体Lipofectamine 2000将anti-miR-222和anti-NC分别转染至T98G细胞后,分别用0、100、200、400和800μmol/L的TMZ处理转染T98G细胞,运用Northern blot等检测miR-222表达水平;以单细胞凝胶电泳(SCGE)检测DNA损伤指标彗性尾长。结果 TMZ引起T98G细胞DNA损伤指标彗星尾长增加,且有一定的剂量效应关系;anti-miR-222处理T98G细胞的DNA损伤指标彗星尾长显著高于同一剂量处理的anti-NC细胞。结论反义抑制miR-222表达可增强替莫唑胺所致神经胶质瘤细胞DNA损伤。Objective To investigate the DNA damage response to temozolomide（ TMZ） in glioma cells with low level expression of miR-222（ T98G-miR-222-）. Methods Anti-miR-222 and anti-NC were transfected into T98 G cells separately by using liposome Lipofectamine 2000. Transfected cells were cultured with 0,100,200,400 and 800 μmol/L TMZ. Expression of miR-222 was analyzed by using Northern blot assay. Single cell gel electrophoresis（ SCGE） was used to analyze the DNA damage of each group. Result The average tail length of DNA comet in glioma cell line induced by TMZ increased and there existed certain dose-response relationship. The average tail length of anti-miR222 group was significantly higher than that of anti-NC group（ P〈0. 05）. Conclusion Anti-sense inhibition of miR-222 expression can enhance DNA damage induced by temozolomide in glioma cells.

关 键 词：MIR-222 反义抑制 替莫唑胺 DNA损伤 

分 类 号：R994.6[医药卫生—毒理学]