二甲基甲酰胺对HL7702肝细胞毒性及线粒体膜电位的影响  

Effect of N,N-dimethylformamide on cytotoxicity and mitochondria membrane potential in HL7702 cells

作　　者：张蔓[1] 管明月[1] 吴智君[1] 郑敏[1] 赵文锦[1] 王磊[1] 程娟[1] 

机构地区：[1]中国疾病预防控制中心职业卫生与中毒控制所,北京100050

出　　处：《毒理学杂志》2017年第4期283-285,300,共4页Journal of Toxicology

基　　金：国家自然科学基金(81372963)

摘　　要：目的观察二甲基甲酰胺(DMF)对HL7702肝细胞的细胞活力、凋亡及线粒体膜电位的影响,探讨DMF引起肝细胞凋亡的可能机制。方法 HL7702肝细胞给予50、100、150、200和250 mmol/L DMF处理,于24 h后检测其细胞存活率,乳酸脱氢酶(LDH)释放量。根据细胞毒性测试结果设置对照组及DMF染毒组(200 mmol/L)。流式细胞术检测DMF对HL7702细胞凋亡以及对线粒体膜电位的影响。结果 DMF剂量依赖性地降低HL7702细胞的存活率,其中150、200和250 mmol/L DMF组与对照组相比,细胞存活率明显降低;并且,随着DMF染毒浓度增加,释放至细胞外的LDH的含量增加,与DMF浓度呈正相关。200 mmol/L DMF染毒24 h后,与对照组相比,细胞凋亡率增加,差异有统计学意义(P<0.001),线粒体膜电位降低,差异也有统计学意义(P<0.001)。结论 DMF对HL7702细胞的毒性作用具有剂量依赖性,DMF引起HL7702线粒体膜电位的改变与DMF导致细胞凋亡有关。Objective To investigate the cytotoxicity and mitochondria damage of N,N-dimethylformamide（ DMF） to HL7702 cells. Methods HL7702 cells were treated with 50,100,150,200 or 250 mmol/L DMF for 24 hours. Cell toxicities of DMF to HL7702 cells were tested by MTT and Lactate dehydrogenase（ LDH） releasing assays. According to theresult of cytotoxicity,normal control group and DMF（ 200 mmol/L） group were set in this study. Cell apoptosis and mitochondria membrane potential were detected using flow cytometry.Result The cell viability was inhibited in a dose dependent manner. Compared with control group,the cell growth in 150,200,and250 mmol/L DMF groups were significantly decreased. The level of LDH in medium was increased significantly with the concentration increasing,and it displayed a dose dependent manner. Compared with control group,cell apoptosis rate in 200 mmol/L DMF group was significantly increased（ P〈0. 001）,while the mitochondrial membrane potential was significantly decreased（ P〈0. 001）. Conclusion DMF resulted in liver cell toxicity in a dose dependent manner,and DMF caused cell apoptosis via reducing the mitochondrial membrane potential.

关 键 词：二甲基甲酰胺 HL7702细胞 细胞毒性 线粒体膜电位 

分 类 号：R114[医药卫生—卫生毒理学]