2011—2016年云南新城疫病毒感染状况调查  

Epidemiological Survey of Newcastle Disease Virus in Yunnan Province during 2011-2016

作　　者：段博芳[1] 王静静[2] 赵云玲[2] 张凤安 吕嵘 吕艳[2] 郑东霞[2] 曾邦权[1] 赵焕云[1] 相德才 刘华雷[2] 张应国[1] 王志亮[2] 

机构地区：[1]云南省动物疫病预防控制中心,昆明650201 [2]中国动物卫生与流行病学中心,青岛266032 [3]丽江市动物疫病预防控制中心,丽江674100 [4]玉溪市动物疫病预防控制中心,玉溪653100 [5]云南省畜牧兽医科学院,昆明650224

出　　处：《畜牧兽医学报》2017年第9期1711-1717,共7页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：公益性行业(农业)科研专项(201303033)

摘　　要：为了解云南地区新城疫流行现状,于2011—2016年在云南部分地区开展了基于风险的新城疫流行病学调查。调查范围涉及昆明、玉溪、大理、丽江、普洱和曲靖六市共48个采样点,共采集不同种类家禽棉拭子样品2 565份。结果显示:共分离到新城疫病毒52株,来源于17个活禽交易市场,新城疫病毒个体阳性率为2.03%,场点阳性率为35.41%。52株新城疫病毒中17株为强毒株,来源于7个农贸市场,新城疫强毒株个体阳性率为0.66%,强毒株场点阳性率为14.58%。遗传演化分析表明:52株新城疫病毒可分为ClassⅠ和ClassⅡ两类,其中ClassⅠ17株,均属于基因3型;ClassⅡ共35株,可分为4种基因型,其中基因Ⅰ型2株,Ⅱ型16株,Ⅵ型13株,Ⅶ型4株。分析表明近年来云南地区流行的新城疫病毒具有多样性,其中基因Ⅵ型主要在鸽群流行,而Ⅶb和Ⅶh亚型则主要在鸡和鹅群中流行。值得关注的是在云南地区首次分离到2株Ⅶh新城疫病毒,而这种基因Ⅶh型的病毒一直在越南等东南亚地区呈地方流行。因此,在该地区应该进一步加强主动监测,分析这种新传入基因型新城疫病毒的分布,防止病原进一步扩散。Risk-based epidemiological survey was conducted in Yunan Province during 2011--2016 in order to better understand the epidemiological relations and their genetic diversity among the circulating Newcastle disease virus （NDV） in this region. A total of 2 565 swab samples were collected from 48 sampling units in Kunming, Yuxi, Dali, Lijiang, Pu＇er and Qujing city. Resultswere as follows： Virus isolation and sequencing were used to determine the molecular characterization of the isolates. Fifty-two NDVs were isolated from samples collected from 17 live bird markets. The individual positive proportion of NDV was 2.03%, and the population positive pro- portion was 35.41%. Among 52 NDV isolates, 17 NDVs isolated from 7 retail markets were proved to be virulent strains based on the sequence analysis of the Fusion gene. The individual positive proportion of virulent NDV was 0. 66%, the population positive proportion of virulent NDV was 14.58%. Phylogenetic analysis revealed that all 52 NDV isolates were clustered into two classes, namely Class Ⅰ and Class Ⅱ. All 17 Class Ⅰ NDV isolates were belonged to geno- type 8. Thirty-five Class U NDV isolates comprised 4 different genotypes, namely genotype Ⅰ （2 isolates）, Ⅱ （16 isolates）, Ⅵ （13 isolates）, Ⅶ （4 isolates）. The genetic diversity of circulating NDVs in Yunnan province was proved, all VI NDV isolates were obtained from pigeon, and 4 genotype Ⅶ NDVs were isolates from chicken and goose. Two genotype Ⅶ h NDVs were first iso- lated from Yunnan in 2013 and 2016 respectively. Genotype Ⅶh NDVs has been endemic in some south-east Asian countries, such as Vietnam, Malaysia, Indonesia, etc. Wild birds may play the role for introduction from neighboring countries. So more active surveillance should be conducted to determine the distribution of the new virus and some control measures also need to be used to prevent the transmission of the new NDVs.

关 键 词：新城疫病毒 流行病学调查 遗传演化分析 

分 类 号：S852.659.5[农业科学—基础兽医学]