犬细小病毒CPV-YH毒株的分离鉴定及其基因组变异分析  

Isolation,Identification and Genomic Variation Analysis of Canine Parvovirus CPV-YH Strain

作　　者：孙明[1] 邓小雨[1] 刘巧荣[1] 马永缨 张丽[1] 卢会英[1] 刘伯华[1] 杨欣艳[1] 陈西钊[1,2] 

机构地区：[1]北京世纪元亨动物防疫技术有限公司,北京100085 [2]中国动物疫病预防控制中心,北京100125

出　　处：《畜牧兽医学报》2017年第9期1724-1729,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金："十三五"国家重点研究计划(2016YFD0501006)

摘　　要：为分析当前犬细小病毒(canine parvovirus,CPV)基因组遗传变异情况,本研究从细小病毒阳性患犬粪便中分离到一株病毒,经过病毒的形态学观察、血凝试验、动物回归试验以及分子生物学鉴定,分离的病毒确实为犬细小病毒,并命名为CPV-YH。病毒分离结果显示,病料接种A-72细胞能产生典型的细胞病变,病毒能凝集猪红细胞,在电子显微镜下呈圆形或六边形,无囊膜,直径约为20nm。动物回归试验复制出了犬细小病毒病典型症状:出血性肠炎、肝水肿、肺淤血。全基因组序列测定与分析显示,其基因组全长为4 921nt,与2011年兰州分离的CPV-LZ2分离株基因组相似性最高,为99%;VP2氨基酸进化树显示,CPV-YH分离株与2013-BJ-P27(中国)分离株和UY306(乌拉圭)分离株在同一个小的分支上。与GenBank上收录的CPV代表株的VP2基因比对,核苷酸和氨基酸相似性分别是98%~99.4%和97.1%~99.5%。以上试验结果表明,成功分离一株CPV变异株,这些数据将为CPV的流行情况和防控提供基础。To analyze genetic variation of canine parvovirus （CPV）, a new virus strain was isolated from the feces of a dog infected by CPV. The virus strain, named as CPV-YH, was identified by red cell agglutination assay, morphology, artificial infection of Beagle dogs and molecular biology. The results showed that the isolate induced specific CPE in A-72 cells, and it could agglutinate porcine red blood cells. The virus were round or hexagonal by the electron microscope, and there was no capsule, with a diameter of about 20 nm. Animal regression test showed that the infected dogs had typical symptoms of CPV disease such as hemorrhagic enteritis, liver edema, pulmonary hyperaemia. The complete genome of CPV-YH was 4 921 nt and it shared the highest nucleotide identity （99%） with CPV-LZ2 isolates isolated from Lanzhou in 2011. The amino acid phylogenetic tree of VP2 showed that CPV-YH isolate, 2013-BJ-P27 （China） and UY306 （Uruguay） isolates were on the same small branch. Compared with the CPV strains on GenBank, the homology of VP2 gene nucleotide and amino acid showed 98%-99.4% and 97.1%-99.5% identities, respectively. In conclusion, one CPV mutant strain was successfully isolated, and these data would provide valuable theoretical basis for the epidemic situation and prevention and control of CPV.

关 键 词：犬细小病毒 分离鉴定 序列分析 

分 类 号：S852.659.2[农业科学—基础兽医学]