雷公藤内酯醇对肝癌H22细胞增殖凋亡及COX-2表达的影响  被引量:4

Effects of Triptolide on Proliferation,Apoptosis,and Cycloxygenase-2 Expression in Hepatic Cancer Cell Line H22

在线阅读下载全文

作  者:甘陈灵 邹玉莲 黄秀旺[1] 许建华[1] 

机构地区:[1]福建医科大学药学院,福州350122 [2]福建医科大学免疫治疗研究所,福州350122

出  处:《福建医科大学学报》2017年第3期146-149,165,共5页Journal of Fujian Medical University

基  金:福建省自然科学基金(2017J01824);福建医科大学苗圃基金(2015MP009)

摘  要:目的观察雷公藤内酯醇(TPL)对肝癌H22细胞增殖、凋亡及环氧合酶-2(COX-2)表达水平的影响。方法以体外培养的鼠源性H22细胞株为研究对象,实验分对照组和不同浓度的TPL处理组。MTT比色法测定TPL对H22细胞株增殖抑制情况;TPL处理48h后,Annexin V-FITC/PI双标记流式细胞术检测细胞凋亡情况,流式细胞术间接免疫荧光双标记法检测COX-2表达变化情况。结果随着药物浓度的增加及时间的延长,TPL能明显抑制H22细胞增殖。TPL诱导细胞凋亡及下调COX-2的表达具有浓度依赖性,不同浓度组比较,差别具有统计学意义(P<0.05)。结论 TPL可能通过诱导肝癌H22细胞凋亡及抑制COX-2表达发挥抗肿瘤作用。Objective To investigate the effects of triptolide(TPL)on proliferation,apoptosis,and cycloxygenase-2(COX-2)expression in liver cancer line H22 and explore its anti-tumor mechanism.Methods H22 cultured in vitro was treated with different concentrations of triptolide,cell proliferation activity was determined by MTT assay. The Annexin V-FITC/PI cell apoptosis and COX-2expression were assessed at 48 hafter the onset of drug treatment by flow cytometry. Result Triptolide significantly inhibited the proliferation of H22 cells in a dose-and time-dependent manner. In addition,triptolide significantly induced cell apoptosis and down-regulated COX-2expression in a dose-manner during48 h. Conclusion TPL may play an anti-liver cancer role by inducing cell apoptosis and inhibiting COX-2expression in H22 cells.

关 键 词:雷公藤内酯 肝肿瘤 细胞凋亡 前列腺素内过氧化物合酶类 

分 类 号:R285[医药卫生—中药学]

 

参考文献:

正在载入数据...

 

二级参考文献:

正在载入数据...

 

耦合文献:

正在载入数据...

 

引证文献:

正在载入数据...

 

二级引证文献:

正在载入数据...

 

同被引文献:

正在载入数据...

 

相关期刊文献:

正在载入数据...

相关的主题
相关的作者对象
相关的机构对象