PCOS与抑郁对雌鼠下丘脑单胺类神经递质和血清黄体生成素、睾酮分泌的影响  

The Influence of PCOS and Depression on the Expression of Neuro-transmitter on Hypothalamic and LH,T in Serum of Female Rats

作　　者：郑珞 陈权[2] 郑志群[1] 

机构地区：[1]福建医科大学附属协和医院妇产科,福州350001 [2]福建医科大学附属协和医院神经外科,福州350001

出　　处：《福建医科大学学报》2017年第3期159-165,共7页Journal of Fujian Medical University

摘　　要：目的研究多囊卵巢综合征(PCOS)及抑郁2种因素对雌性大鼠下丘脑中单胺类神经递质[多巴胺(DA)、去甲肾上腺素(NE)、5-羟色胺(5-HT)、促性腺素释放素(GnRH)]及血清中黄体生成素(LH)、睾酮(T)分泌的影响,分析抑郁与PCOS之间的相互关系。方法将40只雌性6周龄清洁级SD大鼠分为:空白组、抑郁组及PCOS组及PCOS合并抑郁组。采用放射免疫法测定大鼠血清T及LH水平;ELISA法检测大鼠下丘脑中GnRH水平;高效液相色谱-荧光检测流速梯度法检测大鼠下丘脑中NE,DA及5-HT水平。结果 4组大鼠血清LH及T水平、下丘脑GnRH水平比较,差别具有统计学意义(P<0.05),且呈递增趋势。4组大鼠下丘脑的DA,NE及5-HT水平比较,差别具有统计学意义(P<0.05),NE呈递增趋势,DA及5-HT呈递减趋势。GnRH变化趋势与DA(P<0.05,r=-0.874)、5-HT(P<0.05,r=-0.821)变化趋势呈负相关,而与NE变化趋势呈正相关(P<0.05,r=0.809)。结论 PCOS及抑郁2种因素均会使机体中枢DA,NE及5-HT分泌异常,影响下丘脑GnRH、血清LH及T的分泌,影响雌性大鼠的神经内分泌系统,引起机体卵巢组织学、临床表征及心理等发生变化;抑郁可能是PCOS发生及产生远期并发症的重要诱因,而PCOS又可导致抑郁的发生及加剧其发展。Objective To study the impacts of PCOS and depression on serum sex hormone and Neuro-transmitter. To analyze the relationship between depression and PCOS,and to provide the reference for clinical research. Method Forty female SD rats were divided into four groups：normal control group,depression group,PCOS group,and depression with PCOS group. Serum T and LH levels were measured by radioimmunoassay. The levels of GnRH,NE,DA and 5-HT in hypothalamus were measured by ELISA and HPLC fluorescence. Results The difference in rats serum LH and T levels were statistically significant among the four groups（P〈0.05）,and there was a gradual increasing trend. The difference in GnRH levels of rats in the hypothalamus among the four groups had statistical significance（P〈0.05）,and there was a gradual increasing trend. DA,NE and 5-HT levels in hypothalamus were significantly different among 4groups（P〈0.05）,while the NE level showed a increasing trend,whereas DA and 5-HT showed a decreasing trend. Correlation analysis showed that the level of GnRH in each group had a positive correlation with NE（P〈0.05,r=0.809）. The level of GnRH was negatively correlated with DA（P〈0.05,r=-0.874）and 5-HT（P〈0.05,r=-0.821）. Conclusion PCOS and depression are two key factors that may affect the neuroen-docrine system in SD female rats. The abnormal secretion of DA,NE and 5-HT may affect the secretion of hypothalamic GnRH,serum LH and T therefore induce clinical manifestation and psychological and ovarian changes in female rats. Depression may be an important cause of PCOS occurrence and long-term complications,while PCOS can also lead to the occurrence of depression and aggravate its development.

关 键 词：多囊卵巢综合征 抑郁 神经递质药 促性腺素释放激素 性腺甾类激素 

分 类 号：R711.75[医药卫生—妇产科学] R749.4[医药卫生—临床医学]