一测多评法测定华佗豆4种生物碱  

Quantitative analysis on contents of four alkaloid components in Calonyction muricatum by QAMS

作　　者：陈超[1] 周福军[2] 单淇[2] 华洁[2] 王淼[2] 侯文彬[2] 

机构地区：[1]天津医科大学,天津300070 [2]天津药物研究院,天津300193

出　　处：《药物评价研究》2017年第7期938-941,共4页Drug Evaluation Research

摘　　要：目的建立一测多评法检测华佗豆药材中4种生物碱的量。方法以华佗豆碱乙为内标,建立华佗豆碱甲、碱丙、碱丁的相对校正因子,在考察相对校正因子的重现性后,进一步采用外标法与一测多评法所计算出的成分含量进行比较。结果6批华佗豆药材中,各成分采用相对因子计算的量与采用外标法计算的结果无显著差异。结论一测多评法操作简单、结果准确、可靠,可用于华佗豆4种生物碱的测定。Objective To develop a method of quantitative analysis of multi-components by single marker （QAMS） for simultaneously determining four compounds in Calonyction muricatum. Method An HPLC method was developed as QAMS to determine ipalbidine, ipalbidinium, and ipalbinium in C. muricatum, using ipalbine as the intermal reference substance, the relative correction factor （RCF） of the three components was determined by HPLC with good reproducibility. Their contents in six batches of samples were determined by both external standard method and QAMS. Result No significant differences were found in the quantitative results of three alkaloid compounds in six batches of C. muricatum determined by external standard method and QAMS. Conclusion It is feasible and suitable to evaluate the quality ofcurcuma aromatic by QAMS.

关 键 词：一测多评 华佗豆 生物碱 HPLC 华佗豆碱甲 华佗豆碱乙 华佗豆碱丙 华佗豆碱丁 

分 类 号：R917[医药卫生—药物分析学]