姜黄素对小鼠皮肤移植排斥反应的影响  

Immunosuppressive effect of curcumin on skin transplantation in mice

作　　者：张红果[1] 曹军平[2] 树俊莲[1] 刘文霞[3] 马毅[4] 

机构地区：[1]武警总医院药剂科,北京100039 [2]武警总医院消毒供应科,北京100039 [3]武警北京总队第三医院特诊科,北京100141 [4]武警总医院磁共振科,北京100039

出　　处：《药物评价研究》2017年第8期1082-1085,共4页Drug Evaluation Research

摘　　要：目的探讨姜黄素对小鼠皮肤移植排斥反应的免疫抑制作用。方法 BALB/c小鼠作为供体,C57BL/6小鼠作为受体,建立背-背皮肤移植模型。术后分为假手术组、模型组、姜黄素(50 mg/kg)组、环孢素A(10 mg/kg)组、姜黄素+环孢素A(50 mg/kg+5 mg/kg)组,每组10只,每天1次,ip给药10 d。记录各只小鼠移植皮片的存活天数,术后第4、8天采用ELISA法检测血浆白细胞介素-2(IL-2)浓度。结果姜黄素组、环孢素A组、姜黄素+环孢素A组的小鼠移植皮片存活时间分别为14.77、16.81、19.96 d,与模型组(12.10 d)比较差异显著(P<0.01);姜黄素+环孢素A组的小鼠移植皮片存活时间与姜黄素或环孢素A组比较显著延长(P<0.05、0.01);姜黄素组、环孢素A组、姜黄素+环孢素A组术后第4天血浆IL-2水平分别为3.68、2.05、2.70 ng/m L,与模型组(4.76 ng/m L)比较差异显著(P<0.05、0.01);姜黄素组、环孢素A组、姜黄素+环孢素A组术后第8天血浆IL-2水平分别为4.06、2.11、2.95 ng/m L,与模型组(5.85 ng/m L)比较差异显著(P<0.01)。结论姜黄素具有抑制皮肤移植小鼠免疫排斥反应的作用。Objective To investigate the immunosuppressive effect of curcumin on xenogenic skin transplantation in mice. Methods The skin transplantation model was established by an operation, tergal skin flaps from BALB/c donor mice transplanted to the back of C57BL/6 recipient mice. Then recipient mice were divided into five groups at random, namely sham, model, curcumin （50 mg/kg）, Cyclosporin A （10 mg/kg） and curcumin ＋ Cyclosporin A （50 mg/kg ＋ 5 mg/kg）. All mice were ip administered once daily for 10 d. The survival days of skin graft were recorded in all groups. The interleukin：2 （IL-2） levels in plasma of all mice were determined by ELISA 4 and 8 d after the operation, respectively. Results The mean survival time of skin graft in curcumin, Cyclosporin A and curcumin ＋ Cyclosporin A groups were 14.77, 16.81 and 19.96 d, respectively, which showed significant differences comparing with 12.10 d of model group （P 〈 0.01）. Combination of curcumin and Cyclosporin A administration showed a longer mean survival days than curcurni＇n or Cyclosporin A group （P 〈 0.05 or 0.01）. The IL-2 levels in plasma of mice in curcumin, Cyclosporin A and curcumin ＋ Cyclosporin A groups on postoperative day 4 were 3.68, 2.05 and 2.70 ng/mL, respectively, which were significantly reduced than 4.76 ng/mL of model group （P 〈 0.05 or 0.01）. The IL-2 levels in plasma of mice in curcumin, Cyclosporin A and combination of curcumin and Cyclosporin A groups on postoperative day 8 were 4.06, 2.11 and 2.95 ng/mL, respectively, which were significantly reduced than 5.85 ng/mL of model group （P 〈 0.01）. Conclusion Curcumin may have a good immunosuppressive effect on mice with xenogenic skin transplantation.

关 键 词：姜黄素 免疫抑制 皮肤移植 小鼠 白细胞介素-2 

分 类 号：R965.1[医药卫生—药理学]