外源亚精胺对不同干旱胁迫程度下红椿幼苗生理功能的修复调节  

Repair regulation of exogenous Spd on physiological function of Toona ciliata seedlings under drought stress with different levels

作　　者：刘球[1,2] 李志辉[1] 吴际友[2] 杨硕知[2] 吴志华[3] 

机构地区：[1]中南林业科技大学林学院,湖南长沙410004 [2]湖南省林业科学院珍贵树种研究所,湖南长沙410004 [3]国家林业局桉树研究开发中心,广东湛江524022

出　　处：《中南林业科技大学学报》2017年第7期66-72,共7页Journal of Central South University of Forestry & Technology

基　　金：国家林业公益性行业科研专项(201504301)

摘　　要：本研究旨在探讨持续干旱胁迫下外源亚精胺对红椿幼苗生理功能的修复调节效应。以2年生红椿家系盆栽幼苗为试验对象,采取持续干旱和土壤水分测定相结合的方法,在人工遮雨棚中开展干旱胁迫及外源亚精胺调节试验,包括4个处理,即对照处理(土壤相对含水率45%~50%)、轻度干旱胁迫及调节(土壤相对含水率30%~38%+1 mmol/L外源Spd调节)、中度干旱胁迫及调节(土壤相对含水率25%~30%+1 mmol/L外源Spd调节)和重度干旱胁迫及调节(土壤相对含水率20%~25%+1 mmol/L外源Spd调节)。结果表明:(1)在对照、干旱胁迫(轻度、中度和重度)以及喷施外源Spd调节三者之间,红椿幼苗叶片相对电导率、叶绿素含量、相对含水量、MDA含量、POD活性以及游离脯氨酸含量均出现了极显著(p<0.01)差异。(2)1 mmol/L外源Spd喷施修复重度干旱胁迫下植株形态损伤的速度远低于轻度和中度干旱胁迫植株;(3)喷施1 mmol/L Spd可迅速修复红椿的轻度和中度干旱胁迫损伤并提高植株的持续抗旱能力,但其修复重度干旱胁迫损伤作用效果稍弱,但仍具有较强的胁迫指示响应。在干旱胁迫下,1 mmol/L Spd通过提高叶片保水能力和叶绿素含量,激发抗氧化酶活性,减少细胞内物质的外渗,从而增强了红椿幼苗植株抵抗干旱胁迫的能力。The study was carried out to find out the repair regulation effects of exogenous Spd on physiological function of Toona ciliata family seedlings under drought stress. 2a potted seedlings of Toona cilliate adoppted as experimental materials, the drought stress and exogenous Spd regulation experiment was carried out in the artificial-rain-shelter by combining persistent drought stress with soil moisture measurement. The experiment contained 4 treatments as CK （Relative Soil Water Content 45%-50%）, mild drought stress and regulation （Relative Soil Water Content 30%-38%＋1 mmol/L exogenous Spd）, moderate drought stress and regulation （Relative Soil Water Content 25%-30%＋1 mmol/L exogenous Spd） and severe drought stress and regulation （Relative Soil Water Content 20%-25%＋ 1 mmoVL exogenous Spd）. （1）There was extremely significant （p 〈 0.01） difference in leaf relative water content, leaf cell membrane permeability, chlorophyll content, POD content, proline content and MDA content among CK, drought stress（mild, moderate and severe drought stress） and exogenous Spd regulation treatment. （2）The repair effect of 1 mmol/L exogenous Spd on morphologic damage in plants under severe drought stress was much greater than those under mild and moderate drought stress.（3） 1 rnmol/L exogenous Spd treatment could quickly repair damage of plants from mild （Relative Soil Water Content 30%~38%） and moderate （Relative Soil Water Content 25%-30%） drought stress and promote the persistent drought resistance ability, while there was relatively gentle effect on recovery of damage from severe drought stress （Relative Soil Water Content 20%-25%）. 1 mmol/L exogenous Spd treatment could strengthen the drought resistance ability of Toona ciliata seedlings by promote leaf water retaining capacity and chlorophyll content, activating antioxidant enzyme activity and weakening the leakage from cells.

关 键 词：外源亚精胺 生理调节 红椿 干旱胁迫 抗旱能力 

分 类 号：S792.99[农业科学—林木遗传育种]