远志散对东莨菪碱致记忆获得性障碍模型小鼠行为学和脑内AchE活性的影响  

Effects of Yuanzhi San on Ethology and Cerebral Acetylcholinesterase Activity of Memory Disorder Mouse Model Induced by Scopolamine

作　　者：李斌[1] 孙中莉[1] 陈冠儒[1] 郑育卿[1] 何小静 李桂梅 杨榕 邹思政 陈力文 LI Bin SUN Zhong-Li CHEN Guan-Ru ZHENG Yu-Qing HE Xiao-Jing LI Gui-Mei YANG Rong ZOU Si-Zheng CHEN Li-Wen(Chengdu University of Traditional Chinese Medicine, Chengdu 610075 Sichuan, China)

机构地区：[1]成都中医药大学,四川成都610075

出　　处：《广州中医药大学学报》2017年第5期733-736,共4页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：四川省科技创新苗子重点项目(编号:2017RZ0043);成都中医药大学科技发展基金青年教师创新专项(编号:ZRQN1624);成都中医药大学附属医院科技发展基金(编号:2016-D-YY-36)

摘　　要：【目的】观察远志散对东莨菪碱致记忆获得性障碍模型小鼠行为学及脑内乙酰胆碱酯酶(AchE)活性的影响。【方法】将60只昆明种小鼠按体质量随机分为空白组,模型组,阳性药组,远志散低、中、高剂量组。除空白组和模型组给予等体积生理盐水,其他各组给予相应药物10 d后,腹腔注射东莨菪碱(3 mg/kg)复制记忆获得性障碍模型。造模结束后,采用Morris水迷宫和跳台试验观察小鼠学习记忆能力,行为学测试结束后检测各组小鼠脑内AchE活性。【结果】与模型组比较,各治疗组均可缩短模型小鼠Morris水迷宫试验逃避潜伏期(P<0.05),增加有效区停留时间及游泳路程(P<0.05),延长跳台试验的潜伏期(P<0.05),降低模型小鼠脑内AchE活性(P<0.05)。各治疗组组间比较,差异均无统计学意义(P>0.05)。【结论】远志散对东莨菪碱致记忆获得性障碍模型小鼠的学习记忆能力有一定的改善作用,可能与抑制脑内AchE活性有关。Objective To observe the effects of Yuanzhi San （YZS） on the ethology and cerebral acetylcholinesterase （AchE） activity of mouse model of memory disorder induced by scopolamine. Methods Sixty mice were randomly divided into six groups, namely blank control group, model group, positive medicine group, and low-dose, middle-dose and high-dose YZS groups. Except for the blank control group and model group were given the normal saline, the mice in other groups were administered with the corresponding drugs for 10 days. And then, mice in the medication groups were given subcutaneous injection of scopolamine in the dose of 3mg/kg to induce memory disorder model. Morris water maze test and step-down test were adopted for the observation of the learning-memory ability of the mice, and at the end of the tests, the activity of AchE in mouse cerebral cortex was measured by a biochemical method. Results Compared with the model group, escape latency was decreased, and retention time and swimming distance in the effective area in Morris water maze test were prolonged in YZS groups （P 〈 0.05） ; latency in step-down test was also prolonged （P 〈 0.05）. YZS had an effect on decreasing AchE activity in the cerebral cortex of model mice （P 〈 0.05 compared with the model group）. However, the differences were insignificant among the medication groups（P〉 0.05）. Conclusion YZS exerts certain effect on improving learning-memory ability of memory disorder mice induced by scopolamine, and the mechanism might be related with the inhibition of AchE activity in the cerebral cortex of model mice.

关 键 词：远志散/药理学 阿尔茨海默病/中药疗法 行为学 乙酰胆碱酯酶 疾病模型 动物 小鼠 

分 类 号：R285.5[医药卫生—中药学]