不同分化阶段树突状细胞的弹性模量的研究  

The Young's Modulus of Human Dendritic Cells in Different Differentiation Stages

作　　者：黄文竹 胡文慧 曾柱 

机构地区：[1]贵州医科大学生物与工程学院,贵州贵阳550004 [2]贵州医科大学生物与医学工程重点实验室,贵州贵阳550004

出　　处：《贵州医科大学学报》2017年第9期998-1002,共5页Journal of Guizhou Medical University

基　　金：国家自然科学基金(31260227;11162003;31771014;11762006);贵州省2011协同创新计划(2015-04);贵州省科技创新人才团队(2015-4021);教育部科学技术基金重点项目(210196);贵州省科技厅科技合作项目(LH-2016-7375);贵州医科大学博士启动基金(2014-024;2014-022)

摘　　要：目的:探索人树突状细胞(DCs)在不同分化阶段的生物力学特性。方法:收集人外周血单个核细胞来源的CD14+单核细胞,利用重组人白介素-4(rh IL-4)和重组人粒细胞巨噬细胞集落刺激因子(rh GM-CSF)共同诱导分化为未成熟DCs(imDCs),再利用重组人干扰素-γ(rh IFN-γ)和脂多糖(LPS)诱导imDCs分化成熟为DCs(mDCs);利用原子力显微镜(AFM)检测DCs的杨氏模量,采用细胞渗透脆性实验检测DCs的未破碎细胞百分率。结果:在55 m Osm/kg溶液中,mDCs抗低渗溶液的能力较imDCs差,差异有统计学意义(P<0.05);mDCs的杨氏模量高于imDCs,差异有统计学意义(P<0.001)。结论:在DCs不同分化阶段,imDCs的可塑性高于mDCs,形变能力更强,抗低渗溶液的能力更强。Objective： To study the biomechanical characteristics of human dendritic cells （DCs） in different differentiation stages. Methods： CD14 ＋ peripheral blood mononuclear cells were induced to imDCs by recombinant human interleukin-4 （rhIL-4） and recombinant human granuloeyte-maerophage colony-stimulating factor （rhGM-CSF） , and then induced to mDCs by recombinant human interferon γ （rhIFN-γ） and lipopolysaccharide （LPS） . The Young＇ s modulus of DCs were measured by atomic force microscope （AFM） ; the percentage of non-breaking cells was determined by osmotic fragility test under different osmotic pressure to test osmotic fragility of DCs. Results： In 55 mOsm/kg solution, the ability of mDCs to resist hypotonic solution was lower than that of imDCs （ P 〈 0.05 ） ; The Young＇ s modulus of mDCs were higher than that of imDCs （P 〈 0. 001 ）. Conclusions： In different differentia- tion stages of DCs, the plasticity of imDCs is higher than that of mDCs, imDCs have a stronger deform- ation capability and hypotonic resistance capability.

关 键 词：树突状细胞 生物力学特性 渗透脆性 原子力显微镜 杨氏模量 形变能力 

分 类 号：R392.12[医药卫生—免疫学] Q6-3[医药卫生—基础医学]