冠心康对ApoE^(-/-)动脉粥样硬化小鼠血清血脂及炎性微环境的影响  

Effect of Guanxinkang Decoction on Serum Lipids and Inflammatory Microenvironment in APoE^(-/-) Atherosclerotic Mice

作　　者：徐华英[1] 王建茹[1] 王凤[1] 杜文婷[1] 章怡祎[1] 刘萍[1] 

机构地区：[1]上海中医药大学附属龙华医院,上海200032

出　　处：《中国中医急症》2017年第9期1509-1513,共5页Journal of Emergency in Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81573739);上海市中医药三年行动计划项目(ZY3-CCCX-3-3039)

摘　　要：目的观察冠心康对ApoE^(-/-)动脉粥样硬化小鼠的血清白细胞介素-6(IL-6)、白细胞介素-8(IL-8)、肿瘤坏死因子-α(TNF-α)表达的影响,探讨冠心康抗动脉粥样硬化的作用机制与炎性因子之间的关系。方法以ApoE^(-/-)小鼠给予高脂饲料建立动脉粥样硬化模型。30只ApoE^(-/-)小鼠随机分为模型组、冠心康组、辛伐他汀组;10只同遗传背景的C57BL/6J小鼠作为正常组。冠心康组以冠心康煎剂灌胃,辛伐他汀组以辛伐他汀水溶液进行灌胃。各组干预12周时取材。每周检测小鼠体质量;生化仪检测血清血脂;酶联免疫吸附反应(ELISA)检测血清IL-6、IL-8、TNF-α蛋白的表达情况。免疫组化法检测小鼠主动脉CD68蛋白的检测。结果干预12周后,用药组的小鼠体质量均低于模型组(P<0.05)。与模型组比较,冠心康组与辛伐他汀组的血清TC、TG、LDL-C下降(P<0.05),HDL-C升高(P<0.05)。用药组的血清IL-8、TNF-α表达比模型组均下降,差异有统计学意义(P<0.05);IL-6表达则升高(P<0.05)。与模型组比较,用药组的CD68蛋白表达下降,差异有统计学意义(P<0.05)。结论冠心康可能通过调节血清血脂及炎性因子的表达,降低血管内皮细胞的损伤,从而发挥抗动脉粥样硬化的作用。Objective： To observe the effect of Guanxinkang Decoction on the serum lipids and the expression of IL-6,IL-8 and tumor necrosis factor-α（TNF-α） in APoE^（-/-） atherosclerotic mice,and to investigate the relationship between anti-atherosclerosis mechanism and inflammatory factors of Guanxinkang Decoction. Methods：APoE^（-/-） mice were given high fat diet to establish atherosclerosis model. Thirty APoE^（-/-） mice were randomly divided into the model group,traditional Chinese medicine group and western medicine group. Ten C57BL/6J mice with same genetic background were used as the normal group. TCM group was treated with Guanxinkang Decoction,and the western medicine group was fed with simvastatin aqueous solution. Each group was drawn at 12 weeks of intervention. Mouse weight was measured weekly. Detection of serum lipids by biochemical analyzer. The expres-sion of IL-6,IL-8 and TNF-α in serum was detected by enzyme-linked immunosorbent assay（ELISA）. CD68 protein in mouse aorta was detected by immunohistochemistry. Results： After 12 weeks of intervention,the body weight of the mice in the treated groups was lower than that in the model group（P 0.05）. Compared with the model group,the serum levels of TC,TG and LDL-C in the traditional Chinese medicine group and the western medicine group decreased（P 0.05） and HDL-C increased（P 0.05）. The difference was statistically significant（P 0.05）. The expression of IL-6 was increased（P 0.05）,and the expression of IL-8 and TNF-α in the treated groups decreased（P 0.05）. Compared with the model group,the expression of CD68 protein in the treated groups decreased,and the difference was statistically significant（P 0.05）. Conclusion： Guanxinkang Decoction may regulate the expression of serum lipids and inflammatory factors,reduce the damage of vascular endothelial cells,and thus play the role of anti-atherosclerosis.

关 键 词：动脉粥样硬化 冠心康 炎性因子 肿瘤坏死因子-α(TNF-α) 

分 类 号：R285.5[医药卫生—中药学]