从鼠类中检出博卡样病毒及其系统发育分析  

Detection and phylogenetic analysis of Bocavirus from rats

作　　者：姜陆[1] 王刚 谭洋 程晓兰[1] 高玉峰[1] 姚李四[4] JIANG Lu WANG Gang TAN Yang CHENG Xiao-lan GAO Yu-feng YAO Li-si(Liaoning International Travel Healtheare Center, Dalian, Liaoning 116001, China)

机构地区：[1]辽宁出入境检验检疫局,辽宁大连116001 [2]浙江出入境检验检疫局 [3]黑龙江出入境检验检疫局 [4]中国检验检疫科学研究院

出　　处：《中国国境卫生检疫杂志》2017年第4期261-265,共5页Chinese Journal of Frontier Health and Quarantine

摘　　要：目的对我国啮齿类动物博卡病毒的感染情况展开调查。方法 2011—2015年,分别从辽宁大连、丹东、东港,内蒙古满洲里,西藏樟木和新疆哈密境内采集鼠类样本,采用巢氏PCR方法扩增博卡病毒NS1片段和鼠类样本的Cytb片段。结果分别从黑家鼠、褐家鼠、黑线姬鼠、黑线仓鼠、小家鼠、黄胸鼠以及大沙鼠体内检测出博卡病毒65份。系统进化分析表明,鼠博卡病毒同已发现的其他博卡病毒属于不同的进化分支。通过对65个NS1蛋白序列分析,发现其中2个进化支氨基酸一致性小于85%。结论可能存在2个不同的病毒种类,揭示在啮齿动物中博卡病毒有较高的多样性。Objective To investigate Bocavirus infection in the rats in China. Methods The rats were captured from Dalian,Dandong,Donggang,Manzhouli,Zhangmu and Hami during 2011-2015. NS1 gene fragment of Bocavirus and rats Cytb fragment were amplified by using nest PCR method. Results There were 65 Bocavirus had been detected from Rattus norvegicus,Mus musculus,Apodemus agrarius,Cricetulus Barabensis,Rattus flavipectus,Rattus rattus and Rhombomys opimus. Phylogenetic analysis indicated that the rat Bocaviruses had a different evolutionary branch with other Bocaviruses that had been found before. The 65 sequences of NS1 protein were analyzed,it was found that two branch of the evolved amino acids were less than 85%. Conclusion There might be two different Bocavirus species,which revealed a high diversity of bocavirus in rats.

关 键 词：博卡病毒 鼠类 多样性 

分 类 号：R183.5[医药卫生—流行病学]