预设计锚蛋白重复蛋白及其应用进展  

Designed Ankyrin Repeat Proteins and Development of its Application

作　　者：余潇潇 宋潇达[1] 陈晓菲[1] 田浤[1] 高向东[1] YU Xiao-xiao SONG Xiao-da CHEN Xiao-fei TIAN Hong GAO Xiang-dong(Jiangsu Key Laboratory of Druggability of Biopharmaceuticals, School of Life Science and Technology, China Pharmceutical University, Nanjing 210009, China)

机构地区：[1]中国药科大学江苏省生物药物成药性研究重点实验室,生命科学与技术学院,江苏南京210009

出　　处：《药物生物技术》2017年第4期349-352,共4页Pharmaceutical Biotechnology

基　　金：江苏省自然基金青年基金(No.BK20150699);中国药科大学中央高校基本科研业务费专项资金资助(No.2015PY006)

摘　　要：预设计锚蛋白重复蛋白DARPins(Designed ankyrin repeat proteins),是一类衍生自天然锚蛋白重复序列的新型结合蛋白。由于其分子质量小,高效力,高稳定性,高亲和力和具备柔性结构等诸多特点而广受关注。DARPin蛋白在生化研究和临床治疗领域有着极大的应用价值。从DARPin结构的发现到DARPin药物候选分子进入临床,已有十几年的时间。该文总结了DARPin结构与功能特点,并阐述了其在生物医药与免疫治疗领域的应用情况,以期为相关领域的科研人员提供有价值的参考。DARPins is a kind of novel binding protein derived from natural ankyrin repeat sequences. It is popular for its small molecular weight,high efficiency,high stability,high affinity and flexible structure. DARPins have a great application value in biochemical research and clinical treatment. It has been more than ten years from the discovery of DARPin structure to clinical application. This paper summarizes the structure and function of DARPins,and expounds its application in biomedical and immunotherapy to provide more valuable reference for researchers in related fields.

关 键 词：设计锚蛋白重复蛋白 骨架蛋白 类抗体蛋白 小分子蛋白 结构与功能 免疫治疗 靶向治疗 

分 类 号：Q51[生物学—生物化学]