基于12S rDNA和16S rDNA部分序列对澜沧江流域景洪段5种鲃亚科(Barbinae)鱼类的进化地位研究  

Phylogenetic Status of Five Species of Subfamily Barbinae in Lancang River of Jinghong Based on Partial Sequences of 12S rDNA and 16S rDNA

作　　者：李云臻 李俊[2] 徐伟江 范丽仙 潘晓赋[3] LI Yun-zhen LI Jun XU Wei-jiang FAN Li-xian PAN Xiao-tu(School of Life Sciences, Engineering Research Center of Sustainable Development and Utilization of Biomass Energy of Ministry Education, Yunnan Normal University, Kunming 650500, China College of Agronomy and Biological Technology, Yunnan Agricultural University, Kunming 650201, China State key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, China)

机构地区：[1]云南师范大学生命科学学院,生物能源持续开发利用教育部工程研究中心,云南昆明650500 [2]云南农业大学农学与生物技术学院,云南昆明650201 [3]中国科学院昆明动物研究所遗传资源与进化国家重点实验室,云南昆明650223

出　　处：《云南师范大学学报（自然科学版）》2017年第5期40-45,共6页Journal of Yunnan Normal University:Natural Sciences Edition

基　　金：国家自然科学基金资助项目(30660022,30960049,31260507)

摘　　要：通过PCR扩增了澜沧江流域景洪段鲃亚科(Barbinae)短吻鱼(Sikukia gudgeri)、黄尾短吻鱼(S.flavicaudata)、中国结鱼(Tor sinensis)、云南四须鲃(Barbodes huangchuchieni)和长臀鲃(Mystacoleucus marginatus)共5种鱼的线粒体12S rDNA和16S rDNA部分片段,并进行了序列测定,基于12S rDNA和16S rDNA重建NJ系统发育树,结果表明:中国结鱼与野鲮亚科(Labeoninae)亲缘关系较近;云南四须鲃与短吻鱼和黄尾短吻鱼有较近的亲缘关系,长臀鲃次之.基于12S rDNA和16S rDNA序列的遗传学特征佐证了鲃亚科不是一个单系群,四须鲃属(Barbodes)为鲃亚科鱼类进化最原始的属这一观点有待商榷.Partial of 12 S rDNA and 16 S rDNA sequences of 5 species cyprinid fishes that belong subfamily Barbinae were amplified using PCR techniques and the sequences were analyzed in the present study.Fishes of Barbinae,including Sikukia gudgeri,S.flavicaudata,Tor sinensis,Barbodes huangchuchieni and Mystacoleucus marginatus were collected from Jing Hong basin of Lancang River,Yunnan province.Based on the reconstructed neighbor-joining （NJ） tree,phylogenetic status of five species fishes were assessed.The results indicated that T.sinensis was genetically more relative to Labeoninae than Subfamily Barbinae.B.huangchuchieni is genetically more relative to S.gudgeri and S.flavicaudatathan M.marginatus.According to the genetic characteristics of 12 S rDNA and 16 S rDNA sequences,subfamily Barbinae is not proved as a monophyletic population.And the point of view that Barbodes is the most ancestral genus in Barbinae that will be needed still assessed.

关 键 词：鲃亚科 12S RDNA 16S RDNA 澜沧江 系统发育地位 

分 类 号：Q959.4[生物学—动物学]