原子力显微镜(AFM)在细菌生物被膜研究中的应用  

Application of atomic force microscopy(AFM) to study bacterial biofilms

作　　者：杨闪闪 黄巧云[1] 蔡鹏[1] 

机构地区：[1]华中农业大学资源与环境学院农业微生物学国家重点实验室,湖北武汉430070

出　　处：《生物工程学报》2017年第9期1399-1410,共12页Chinese Journal of Biotechnology

基　　金：国家重点研发计划(No.2016YFD0800206);国家自然科学基金(No.41522106)资助~~

摘　　要：原子力显微镜(AFM)作为一项重要的表面可视化技术,以其独特的优势(纳米级的空间分辨率、皮牛级力灵敏度、免标记、可在溶液环境下工作)被广泛应用于生物被膜的研究。AFM不仅可以在近生理环境下对生物被膜表面超微形貌进行可视化表征,同时还可以通过纳米压痕对生物被膜的机械特性(弹性和粘性)进行定量测量,利用AFM单细胞和单分子力谱技术可以获得生物被膜形成过程中细胞-基底以及细胞-细胞之间的相互作用力,为生物被膜的实时原位系统研究提供了可行性。本文简述了AFM的基本操作原理,综述了近年来AFM用于生物被膜表面超微结构成像、机械特性测量以及相互作用力研究方面的进展,并对AFM在生物被膜研究中面临的问题和未来的发展方向进行了讨论。Because of the nanometre resolution, piconewton force sensitivity, label-free technique and the ability to operate in liquid environments, atomic force microscopy （AFM） has emerged as a powerful tool to explore the biofilm development processes. AFM provides three-dimensional topography and structural details of biofilm surfaces under in-situ conditions. It also helps to generate key information on the mechanical properties of biofilm surfaces, such as elasticity and stickiness. Additionally, single-molecule and single-cell force spectroscopies can be applied to measure the strength of adhesion, attraction, and repulsion forces between cell-solid and cell-cell surfaces. This paper outlined the basic principleof AFM technique and introduced recent advances in the application of AFM for the investigation of ultra-morphological, mechanical and interactive properties of biofilms. Furthermore, the existing problems and future prospects were discussed.

关 键 词：原子力显微镜 细菌生物被膜 超微形貌 机械特性 相互作用力 

分 类 号：Q934[生物学—微生物学]