川产药用鸡矢藤UPLC指纹图谱研究  

Study on UPLC Fingerprint of Paederiae Herba from Sichuan

作　　者：胡明勋[1] 马俊[1] 吴兵[1] 马逾英[1] 杨洁[1] 兰杨[1] Hu Mingxun Ma Jun Wu Bing Ma Yuying Yang Jie Lan Yang(School of Pharmacy,Chengdu University of Traditional Chinese Medicine/State Key Laboratory breeding Base of Systematic Research,Development and Utilization of Chinese Medicine Resources,Chengdu 61137,China)

机构地区：[1]成都中医药大学药学院中药资源系统研究与开发利用省部共建国家重点实验室培育基地,四川成都611137

出　　处：《亚太传统医药》2017年第18期32-35,共4页Asia-Pacific Traditional Medicine

摘　　要：目的:研究鸡矢藤药材的UPLC指纹图谱。方法:采用Aglient ZORBAX SB-C_(18)(2.1mm×50mm,1.8μm)色谱柱,流动相为乙腈-0.1%甲酸水溶液梯度洗脱,流速为0.25mL/min,检测波长为250nm,柱温为30℃,进样量为1μL。并对测得的指纹图谱进行相似度评价、主成分分析和聚类分析。结果:21批不同样品共确立了9个共有峰,建立了鸡矢藤药材的UPLC对照指纹图谱。通过聚类分析,将鸡矢藤药材分为2类。通过对9个共有峰主成分分析,提取了3个主成分。结论:建立的方法快速简便,精密度、重复性、稳定性良好,可用于鸡矢藤药材的品质分析。Objective：To study UPLC Fingerprint of Paederiae Herba. Methods： UPLC analysis was carried out on an Aglient ZORBAX SB-C18 （2.1mm×50mm, 1.8μm） column with the gradient elution of acetonitrile and 0.1% formic acid. The flow rate, detection wavelength, column temperature and injection volume was 0.25mL/min, 250nm, 30℃ and 1μL, respectively. The similarity evaluation, principal component analysis and cluster analysis were used to analyze UPLC fingerprint of Paederiae Herba. Results：UPLC fingerprint of Paederiae Herba was established including 9 common peaks by analyzing 21 hatches of different samples. Based on cluster analysis, 21 batches of different samples were divided into two categories. Three principal components were extracted by principal component analysis from 9 common peaks. Conclusion： The established UPLC fingerprint method is fast and shows good precision, repeatability and stability. It can be used to investigate Paederiae Herba quality.

关 键 词：鸡矢藤 UPLC 指纹图谱 鸡矢藤苷 

分 类 号：R284.1[医药卫生—中药学]