炮山甲的HPLC指纹图谱研究  

Study on HPLC Fingerprint of Stir-baked Manis pentadactyla

作　　者：严丹[1] 刘玉杰[1] 胡美变 肖禾 李永川 吴纯洁[1,3] 

机构地区：[1]成都中医药大学药学院,成都611137 [2]成都岷江源药业股份有限公司,成都611000 [3]国家中医药管理局中药炮制技术重点研究室,成都611731

出　　处：《中国药房》2017年第27期3839-3842,共4页China Pharmacy

基　　金：国家中医药行业科研专项项目(No.201207004-6)

摘　　要：目的:建立炮山甲的高效液相色谱(HPLC)指纹图谱。方法:采用HPLC法。色谱柱为Capcell Pak MgⅡS5 C18,流动相为乙腈-0.1%磷酸溶液(梯度洗脱),流速为0.8 m L/min,检测波长为275 nm,柱温为30℃,进样量为10μL。以酪氨酸为参照,测定11批药材的HPLC图谱,采用《中药色谱指纹图谱相似度评价系统》(2004 A版)进行共有峰指认和相似度评价。结果:11批炮山甲药材的HPLC图谱有23个共有峰,相似度均>0.90。经验证,11批药材样品HPLC图谱与对照指纹图谱具有较好的一致性。结论:该研究所建HPLC指纹图谱可为炮山甲的鉴别和质量评价提供参考。OBJECTIVE： To establish HPLC fingerprint of stir-baked Manis pentadaetyla. METHODS ： HPLC method was con- ducted. The determination was performed on Capcell Pak Mg ⅡS 5 C18 column with mobile phase consisted of acetonitrile-0.1% phosphoric acid solution （gradient elution） at the flow rate of 0.8 mL/min. The detection wavelength was set at 275 nm, and col- umn temperature was 30℃. The sample size was 10 μL. Using oxyphenylaminopropionic acid as reference, HPLC chromatograms of 11 batches of medicinal materials were determined. TCM Chromatogram Fingerprint Similarity Evaluation System （2004 A edi- tion） was used for common peak identification and similarity evaluation. RESULTS： There were 23 common peaks in 11 batches of stir-baked M. pentadactyla, with similarity〉0.90. After validation, HPLC chromatograms of 11 batches of medicinal materials were in good agreement with control fingerprints. CONCLUSIONS： Established HPLC fingerprint can provide reference for the identification and quality evaluation of stir-baked M. pentadactyla.

关 键 词：炮山甲 高效液相色谱法 指纹图谱 质量评价 

分 类 号：R282[医药卫生—中药学] R917[医药卫生—中医学]