Orexin Directly Enhances the Excitability of Globus Pallidus Internus Neurons in Rat by Co-activating OX1 and OX2 Receptors  

作　　者：He-Ren Gao Qian-Xing Zhuang Yong-Xiao Zhang Zhang-Peng Chen Bin Li Xiao-Yang Zhang Yi-Ting Zhong Jian-Jun Wang Jing-Ning Zhu 

机构地区：[1]State Key Laboratory of Pharmaceutical Biotechnology and Department of Biological Science and Technology,School ofLife Sciences,Nanjing University,Nanjing 210023,China

出　　处：《Neuroscience Bulletin》2017年第4期365-372,共8页神经科学通报（英文版）

基　　金：supported by grants from the National Natural Science Foundation of China(NSFC;31330033,91332124,31471112,31500848,81671107,31600834,J1210026);the NSFC/Research Grants Council(RGC)Joint Research Scheme(31461163001);the Ministry of Education of China(Specialized Research Fund for the Doctoral Program of Higher Education/RGC Earmarked Research Grant 20130091140003,and Fundamental Research Funds for the Central Universities);the Natural Science Foundation of Jiangsu Province,China(BK2011014,BK20140599,and BK20151384);the Postdoctoral Sciences Foundation,China(2013T60520)

摘　　要：Orexin, released from the hypothalamus, has been implicated in various basic non-somatic functions including feeding, the sleep-wakefulness cycle, emotion,and cognition. However, the role of orexin in somatic motor control is still little known. Here, using whole-cell patch clamp recording and immunostaining, we investigated the effect and the underlying receptor mechanism of orexin-A on neurons in the globus pallidus internus（GPi）,a critical structure in the basal ganglia and an effective target for deep brain stimulation therapy. Our results showed that orexin-A induced direct postsynaptic excitation of GPi neurons in a concentration-dependent manner.The orexin-A-induced excitation was mediated via co-activation of both OX1 and OX2 receptors. Furthermore, the immunostaining results showed that OX1 and OX2 receptors were co-localized in the same GPi neurons. These results suggest that the central orexinergic system actively modulates the motor functions of the basal ganglia via direct innervation on GPi neurons and presumably participates in somatic-non-somatic integration.Orexin, released from the hypothalamus, has been implicated in various basic non-somatic functions including feeding, the sleep-wakefulness cycle, emotion,and cognition. However, the role of orexin in somatic motor control is still little known. Here, using whole-cell patch clamp recording and immunostaining, we investigated the effect and the underlying receptor mechanism of orexin-A on neurons in the globus pallidus internus（GPi）,a critical structure in the basal ganglia and an effective target for deep brain stimulation therapy. Our results showed that orexin-A induced direct postsynaptic excitation of GPi neurons in a concentration-dependent manner.The orexin-A-induced excitation was mediated via co-activation of both OX1 and OX2 receptors. Furthermore, the immunostaining results showed that OX1 and OX2 receptors were co-localized in the same GPi neurons. These results suggest that the central orexinergic system actively modulates the motor functions of the basal ganglia via direct innervation on GPi neurons and presumably participates in somatic-non-somatic integration.

关 键 词：OREXIN OX1 receptor OX2 receptor Globus pallidus internus Basal ganglia 

分 类 号：R338[医药卫生—人体生理学]