MIF在新生大鼠缺氧缺血性脑损伤中的表达及定位研究  

The study of MIF expression and celluar localization in the cerebral cortex of neonatal rats with hypoxic-ischemic brain damage

作　　者：宋红花[1] 李海英 SONG Honghua LI Haiying(Laboratory of Paediatrics, the Affiliated Hospital of Nantong University, Jiangsu 226001)

机构地区：[1]南通大学附属医院儿科实验室,江苏226001

出　　处：《交通医学》2017年第3期209-211,215,共4页Medical Journal of Communications

基　　金：江苏省妇幼健康科研项目(F201649);南通市青年基金项目(WQ2016071)

摘　　要：目的:研究巨噬细胞迁移抑制因子(MIF)在新生SD大鼠缺氧缺血性脑损伤(HIBD)大脑皮层中的表达变化及与小胶质细胞的定位关系。方法:建立HIBD模型,采用Western Blot检测缺氧缺血再灌注0h、24h、48h、72h大脑皮层中MIF的表达变化,采用免疫荧光组织染色检测缺氧缺血再灌注48h大脑皮层中MIF表达及与小胶质细胞的定位。结果:在HIBD模型中,MIF表达明显升高,在缺氧缺血再灌注48h时表达丰度最高且与小胶质细胞存在共定位关系。结论:HIBD可诱导大脑皮层中小胶质细胞MIF的表达,MIF的上调可能参与了新生大鼠缺氧缺血性脑损伤的致病过程。Objective：To investigate the expression changes of macrophage migration inhibitory factor（MIF） and its celluar localization with microglia in the cerebral cortex of neonatal rats with hypoxic-ischemic brain damage（HIBD）.Methods： With the establishment of hypoxic-ischemic brain damage model, Western blot was used to study the expression changes of MIF in the cerebral cortex after hypoxia-ischemia reperfusion 0h, 24 h, 48 h and 72 h. Immunohistochemistry method was used to study the celluar localization with microglia in the injured cerebral cortex on 48 h. Results：In the HIBD model, The MIF expression was significantly increased and at its peak on 48 h. Immunohistochemistry demonstrated that MIF was colocalized with microglia. Conclusions：HIBD could activate microglia to express MIF and MIF might be involved in the pathogenesis of HIBD.

关 键 词：缺氧缺血性脑损伤 巨噬细胞迁移抑制因子 大脑皮层 小胶质细胞 大鼠 

分 类 号：R722.12[医药卫生—儿科]