上饶市乙型流感病毒耐药基因分析  

Neuraminidase gene profiling of influenza B virus in Shangrao

作　　者：张艳燕 赖宇 朱琳 熊英[3] 李健雄[3] 程畅河 

机构地区：[1]上饶市疾病预防控制中心检验科,江西上饶334000 [2]江西医学高等专科学校药学系 [3]江西省疾病预防控制中心检验科

出　　处：《中国感染与化疗杂志》2017年第5期538-540,共3页Chinese Journal of Infection and Chemotherapy

基　　金：2014年江西省医学学科省市共建计划项目(201412)

摘　　要：目的分析上饶市乙型流感病毒耐药基因,为临床用药和疾病控制提供依据。方法从哨点医院采集疑似流感患者咽拭子样本,接种犬肾传代细胞(Madin-Darby Canine Kidney,MDCK)及进行分离培养。选择分离到的乙型(即B型)流感病毒株进行核酸提取后进行反转录-聚合酶链反应(RT-PCR),扩增神经氨酸酶(neuraminidase,NA)基因并进行核苷酸测序,采用DNA Star6.0、Mage5.0生物软件对测序结果进行分析处理,推导出氨基酸序列,进行基因特性分析。结果7株乙型流感病毒NA基因同源性高达99.5%以上,NA蛋白催化活性位点和辅助位点均无氨基酸替换。结论 7株毒株均对流感病毒NA抑制剂药物敏感,但仍应加强对流感病毒的耐药性监测。Objective To characterize the neuraminidase （NA） genes of influenza B virus in Shangrao. Methods The specimens of nasopharyngeal swabs were collected from patients with influenza-like symptoms in influenza sentinel hospital. Seven strains of influenza B virus were randomly selected for culture and isolation in Madin-Darby Canine Kidney Epithelial Cells （MDCK Line）. Viral RNA was extracted. Fragments of NA genes were amplified by one-step RT-PCR and then were sequenced. The data obtained were analyzed with software DNAStar 6.0 and Mage 5.0. The deduced amino acid sequences were examined to explore the features of NA gene. Results The NA gene showed high homology of nucleotides between the 7 strains of influenza B virus. No amino acid substitution was found in catalytic or framework residues of the deduced amino acid sequences of NA gene. Conclusions All the 7 strains of influenza B virus were sensitive to neuraminidase inhibitors. However, ongoing resistance surveillance is necessary for control and prevention of influenza.

关 键 词：乙型流感病毒 耐药 神经氨酸酶基因 

分 类 号：R440[医药卫生—诊断学] R511.7[医药卫生—临床医学]