儿童患者下呼吸道标本苛养菌阳性分离率的影响因素分析  

Factors affecting the isolation of fastidious bacteria from lower respiratory tract specimens in children

作　　者：潘芬[1] 赵婉彤 张天栋[1] 石迎迎[1] 蒋婕 张泓[1] 

机构地区：[1]上海市儿童医院,上海交通大学附属儿童医院检验科,上海200040

出　　处：《中国感染与化疗杂志》2017年第5期541-545,共5页Chinese Journal of Infection and Chemotherapy

基　　金：上海申康医院发展中心市级医院临床辅助科室能力建设项目(SHDC22014012);上海市卫计委重要薄弱学科建设(2015ZB0203)

摘　　要：目的分析儿童患者下呼吸道标本苛养菌(肺炎链球菌和流感嗜血杆菌)阳性分离率的影响因素。方法选取210份下呼吸道标本,分别在采集后0.5 h、2 h、6 h、12 h、24 h接种于血平皿和巧克力平皿,研究运送时间、运送培养基和不同接种培养基对苛养菌阳性分离率的影响。结果 200份合格下呼吸道标本研究结果显示:随着标本放置时间的增加,肺炎链球菌和流感嗜血杆菌阳性分离率呈现下降趋势,除0.5 h、2 h时间段外结果差异具有统计学意义(P<0.05),但0.5 h、2 h 2个时间段样本阳性率无差异;含半固体培养基的阳性分离率在不同时间段均高于其他2种运送培养基,但差异并无统计学意义(P>0.05);肺炎链球菌和流感嗜血杆菌在两种商品化平皿中阳性分离率、生长情况差别不大(P>0.05)。结论选择合适的运送时间、运送培养基和接种培养基对于提高苛养菌阳性分离率具有一定的意义。Objective To investigate the factors affecting the isolation of fastidious bacteria such as Streptococcus pneumoniae and Haemophilus influenzae from lower respiratory tract specimens in children. Methods A total of 210 lower respiratory tract specimens from children were collected and inoculated on both blood agar plate and chocolate agar plate 0.5 h, 2 h, 6 h, 12 h, and 24 h after collection. The effect of specimen turnaround time, transport medium, and inoculation medium on the isolation of fastidious bacteria was studied. Results The results of 200 qualified specimens showed that the isolation of S. pneumoniae and H. influenzae significantly decreased with the increasing of specimen turnaround time （P〈~0.05）, but no significant difference was observed between 0.5 h and 2 h timepoints. The isolation of fastidious bacteria in semi-solid medium was non-significantly higher than the other two transport medium （/）〉0.05）. No significant difference was found in the isolation and growth ofS. pneumoniae and H. influenzae on different inoculation media （P〉0.05）. Conclusions The appropriate specimen turnaround time, transport medium, and inoculation medium are important for improving the isolation of fastidious bacteria from lower respiratory tract in children.

关 键 词：下呼吸道 苛养菌 阳性分离率 

分 类 号：R446.5[医药卫生—诊断学] R725.6[医药卫生—临床医学]