四种“蒂达”类藏药提取物对四氯化碳损伤人肝细胞的保护作用研究  

Research on the Protective effects of Four Tibetan medicine “Dida” Extracts on Liver Cell Injure Induced by CCl_4

作　　者：李博 张寿文 李姗姗 曹岚 慕泽泾 杜小浪 钟国跃 蒋伟 

机构地区：[1]江西中医药大学中药资源与民族药研究中心,江西南昌330004 [2]江西民族传统药现代科技与产业发展协同创新中心,江西南昌330004 [3]江西中医药大学实验动物科技中心,江西南昌330004

出　　处：《时珍国医国药》2017年第8期1797-1799,共3页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.81360675);国家科技重大专项子课题(No.2012ZX09103201-050)

摘　　要：目的考察四种"蒂达"类藏药提取物对体外四氯化碳损伤人肝细胞的保护作用,为该类药材的开发利用提供药理学研究依据。方法采用四氯化碳诱导的人肝L-02细胞损伤模型,以四种"蒂达"类藏药提取物进行干预,水飞蓟宾为对照,MTT法测定细胞存活率并测定细胞培养上清液中的谷丙转氨酶(ALT)、谷草转氨酶(AST)的活力、丙二醛(MDA)的含量,以及细胞裂解上清液中的超氧化物气化酶(SOD)的活力。结果四种"蒂达"类藏药提取物均可显著提高肝细胞的存活率,可显著性降低四氯化碳致肝细胞损伤的转氨酶(ALT、AST)的活力,显著升高超氧化物气化酶(SOD)的活力,降低丙二醛(MDA)的含量,其中篦齿虎耳草提取物对上述指标的影响改变尤为显著。结论四种"蒂达"类藏药均可减轻四氯化碳致肝细胞损伤程度,其中篦齿虎耳草效果最为明显,其作用机制与抗自由基而减轻脂质过氧化损伤相关。Objective To investigate protective effects of four Tibetan medicine ＂Dida＂extracts on liver cell injure induced by CCl4,and provide the pharmacological basis for the development of the Tibetan medicine＂Dida＂which has a complicated varieties on the clinical practice. Methods The model of liver cell injure induced by CCl_4 was used with Tibetan medicine＂Dida＂extracts to intervene and Silybin for positive control. The MTT method was adopted to detect the cell activity. Meanwhile,the level or content of AST,ALT and MDA were determined in cell culture supernatant and the level of SOD in cell splitting supernatant.Results Four Tibetan medicine＂Dida＂extracts all significantly improved the cell activity,and increased the levels of ALT and AST in cell culture supernatant,and increased the level of SOD in cell splitting supernatant while significantly decreased the content of MDA in cell culture supernatant. The markers mentioned above were most significantly regulated by Saxifaga umbellulata var petinata. Conclusion Four Tibetan medicine ＂Dida＂extracts can reduce the degree of Liver cell injure induced by CCl4 with different levels and S. umbellulata var. petinata could take best effect. The possible mechanism of ＂Dida＂extracts＇ activity may be relevant to relieve the damage of membrane lipid peroxidation by scavenging free radical.

关 键 词：“蒂达” L-02细胞 肝细胞损伤 

分 类 号：R29[医药卫生—民族医学]