安胃汤对慢性萎缩性胃炎大鼠Fas、Fas-L表达的影响  

Effects of Anwei Decoction on Fas/Fas-L Expression in Rat Models of Chronic Atrophic Gastritis

作　　者：韦维[1] 林寿宁[2] 汪波[1] 万文雅[1] 庞旺风 朱永苹[2] 韦德锋[1] 

机构地区：[1]广西中医药大学,广西南宁530200 [2]广西中医药大学瑞康医院,广西南宁530011

出　　处：《时珍国医国药》2017年第8期1830-1833,共4页Lishizhen Medicine and Materia Medica Research

基　　金：国家自然科学基金(No.81360533);广西自然科学基金(No.2014GXNFSAA118148)

摘　　要：目的从细胞凋亡因子Fas、Fas-L、Bax、Bcl-2角度探讨安胃汤对慢性萎缩性胃炎模型大鼠疗效的作用机制。方法采用甲基硝基亚硝基胍(MNNG)慢性萎缩性胃炎大鼠模型,应用安胃汤进行干预;运用HE染色观察胃黏膜组织形态改变;ELISA方法检测血清中Fas-L含量;采用实时荧光定量PCR方法、Western blot检测Fas、Bax、Bcl-2基因表达及其蛋白含量的水平。结果该方可以明显上调Fas、Bax基因以及其蛋白含量的表达,下调外周血Fas-L的含量和Bcl-2基因以及其蛋白含量的表达。结论安胃汤能够明显改善CAG大鼠胃组织的病理变化,具有明显的抗CAG作用,其作用机制可能通过提高Fas基因及蛋白的表达,降低Fas-L含量,激活CAG大鼠胃黏膜细胞Fas/Fas-L信号传导通路,降低Bcl-2基因及蛋白,升高Bax基因及蛋白,促进胃黏膜细胞凋亡,可能是安胃汤防治CAG的关键机制之一。Objective To explore the mechanism underlying the effects of Anwei Decoction on model rats with chronic atrophic gastritis（ CAG） from the perspective of the Fas/Fas-L signalling pathway. Methods MNNG was used to establish models of CAG rats which were then subject to the intervention by Anwei Decoction; ELISA was employed to measure the serumal Fas L level; quantitative real-time fluorescent PCR and Western blot were used to measure the expression of genes Fas、Bax and Bcl-2and their protein levels. Results Compared with the pathological model group,the Anwei Decoction group had normal gastric tissue structure,more compact and regular glands,and showed no deformation and atrophy. The formula significantly up-regulated the expression of genes Fas,Bax and their protein levels,and down-regulated the level of Fas-L in the peripheral blood serum and the expression of gene Bax and its protein level. Conclusion Anwei Decoction is able to obviously improve the pathological changes of gastric mucosa of CAG rats,has obvious anti CAG effect,its mechanism may be through increasing the expression of Fas gene and protein,decreased the content of Fas-L,activation of gastric mucosal of CAG Fas/Fas-L signaling pathway in rats,reduce Bcl-2 gene and protein,increased Bax gene and protein,promote apoptosis of gastric mucosal cells,may be the key mechanism of Anwei Decoction control of CAG.

关 键 词：安胃汤 慢性萎缩性胃炎 FAS FAS-L 

分 类 号：R285.5[医药卫生—中药学]